CHRIST (Deemed to University), Bangalore

DEPARTMENT OF LIFE SCIENCES

School of Sciences

Syllabus for
Master of Science (Forensic Science)
Academic Year  (2023)

 
1 Semester - 2023 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
MFS131 FORENSIC SCIENCE, PHOTOGRAPHY, CRIME SCENE MANAGEMENT Core Courses 4 4 100
MFS132 CRIMINOLOGY, CRIMINAL LAW AND POLICE ADMINISTRATION Core Courses 4 4 100
MFS133 ANALYTICAL INSTRUMENTS AND TECHNIQUES IN FORENSIC SCIENCE Core Courses 4 4 100
MFS134 FUNDAMENTALS OF QUESTIONED DOCUMENT EXAMINATION Core Courses 4 4 100
MFS135 RESEARCH METHODOLOGY IN FORENSIC SCIENCES Generic Elective Courses 2 2 50
MFS151 PATTERN EVIDENCE AT SCENE OF CRIME, FORENSIC PHOTOGRAPHY AND FORENSIC DOCUMENT EXAMINATION LAB Core Courses 8 4 100
MFS152 ANALYTICAL INSTRUMENTS AND TECHNIQUES IN FORENSIC SCIENCE LAB Core Courses 4 8 100
2 Semester - 2023 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
MFS231 FORENSIC BIOLOGY AND FORENSIC MEDICINE - 4 4 100
MFS232 FORENSIC CHEMISTRY AND TOXICOLOGY - 4 4 100
MFS233 FORENSIC BALLISTICS AND FORENSIC PHYSICS - 4 4 100
MFS234 INFORMATION SECURITY AND CYBER CRIME - 4 4 100
MFS235 ESSENTIALS OF MATHEMATICS AND STATISTICS IN FORENSIC SCIENCE - 3 3 100
MFS251 FORENSIC BIOLOGY, FORENSIC CHEMISTRY, AND TOXICOLOGY LAB - 8 4 100
MFS252 FORENSIC BALLISTICS, FORENSIC PHYSICS AND INFORMATION SECURITY LAB - 8 4 100
3 Semester - 2022 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
MFS331 FORENSIC ANTHROPOLOGY Core Courses 4 4 100
MFS332 FORENSIC DERMATOGLYPHICS AND OTHER IMPRESSIONS Core Courses 4 4 100
MFS333 FORENSIC GENETICS AND FORENSIC SEROLOGY Core Courses 4 4 100
MFS334 FORENSIC DNA PROFILING AND BIOINFORMATICS Core Courses 4 4 100
MFS335 FUNDAMENTALS OF FORENSIC PSYCHOLOGY Core Courses 3 3 100
MFS351 FORENSIC ANTHROPOLOGY LAB Core Courses 4 2 100
MFS352 FORENSIC SEROLOGY, DNA PROFILING AND BIOINFORMATICS LAB Core Courses 4 4 100
4 Semester - 2022 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
MFS441A FORENSIC DRUGS ANALYSIS - 8 4 100
MFS441B FORENSIC BIOLOGY AND WILDLIFE FORENSICS - 4 4 100
MFS441C MODERN TRENDS IN FINGERPRINT SCIENCE - 4 4 100
MFS442A ANALYTICAL FORENSIC TOXICOLOGY - 4 4 100
MFS442B THEORETICAL AND PRACTICAL ASPECTS OF BIOLOGICAL EVIDENCES - 4 4 100
MFS442C ADVANCED QUESTIONED DOCUMENTS - 4 4 100
MFS451A FORENSIC DRUGS ANALYSIS AND ANALYTICAL FORENSIC TOXICOLOGY LAB - 8 4 100
MFS451B FORENSIC BIOLOGY AND WILDLIFE FORENSICS AND THEORETICAL AND PRACTICAL ASPECTS OF BIOLOGICAL EVIDENCES LAB - 8 4 100
MFS451C PRACTICAL OF QUESTIONED DOCUMENTS AND FINGERPRINTS - 8 4 100
MFS481A DISSERTATION - 0 12 150
MFS481B MAJOR PROJECT - 0 12 150
    

    

Introduction to Program:

Forensic Science is the application of a broad spectrum of sciences to answer questions of interest to a legal system. Forensic science uses all the principles of various branches of science and applies them in experiments to draw an exact conclusion. In M Sc.Forensic Sciences, one needs to study the various principles and theories that are applicable in Forensic science. The duration of Master of Science in Forensic Sciences is two academic years. The syllabus for the course is divided into four semesters and it is career orienting in nature.

Programme Outcome/Programme Learning Goals/Programme Learning Outcome:

PO1: Learn and understand the concepts of Forensic science.

PO2: Implement advanced techniques for analysis and examination of crime.

PO3: Design and develop research-based solutions for forensic problems.

PO4: Understand the different crime scenario according to the prevailing legal provisions.

PO5: Apply ethical principles and commit to professional ethics, responsibilities and norms of forensic practices.

PO6: Develop proficiency for employability, entrepreneurship and competitive exams.

Assesment Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMESTER EXAMINATION: Consist of 2 sections. Section A consists of 10 questions carrying 5 marks each out of which students need to attempt 8 questions (8 X 5marks = 40 marks). Section B consists of 7 questions, carrying 12 marks each, out of which students need to attempt 5 questions (5 X 12 marks = 60 marks).

Examination And Assesments

The evaluation scheme for each course shall contain two parts; (a) internal evaluation and (b) external evaluation. 50% weightage shall be given to the internal evaluation and the remaining 50% to external evaluation and the ratio and weightage between internal and external is 1:1.  (a) Internal evaluation: The internal evaluation shall be based on a predetermined transparent system involving periodic written tests, assignments, seminars, and attendance in respect of theory courses and based on written tests, lab skill/records/viva, and attendance in respect of practical courses.

MFS131 - FORENSIC SCIENCE, PHOTOGRAPHY, CRIME SCENE MANAGEMENT (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to introduce the concept of forensic science, its development and progress over time. It will also familiarize students with the organizational setting and working set up of forensic laboratories. It aims to introduce different techniques used in forensic investigations with the principles involved. The course would apprise students with the basics of forensic photography techniques with management of crime scene in different situations.  

Course Outcome

CO1: To understand the basic concept, meaning, significance and development of Forensic science.

CO2: To gain knowledge of various types of tool marks and gemmology along with technological advancement.

CO3: To thoroughly document the entire scene, the evidence, and any areas of special significance to the investigation.

CO4: To describe Crime scene investigations, reconstruction of scene of crime, chain of custody, and its relevance.

CO5: To elucidate research methodologies and techniques used in the formation of research design on a specific problem

Unit-1
Teaching Hours:15
General introduction of Forensic Science
 

Introduction and history and development of forensic science, Laws and Basic principles of Forensic Science, Need Scope, Concepts and Significance of Forensic Science, Branches of forensic science, Forensic Labs in India; Organizational set-up of a Forensic Science Laboratory. The units in a forensic lab; Investigative strategies. Role and responsibilities of forensic expert at crime scene, Expert testimony and eye-witness report.

Unit-2
Teaching Hours:15
Introduction to Tool marks, restoration & forensic Gemology
 

Introduction to tool marks, Toolmark evidence. Classification of toolmarks: compression marks, striated marks, repeated marks, combination of compression and striated marks. Class and individual characteristics of tool marks, Tracing and lifting of marks; Collection, preservation and matching of toolmarks; Photographic examination of tool marks; Forensic importance of toolmarks; Toolmark databases; Types of tool marks: cast, punch, engrave; Methods of 7 obliteration, Principle of Restoration; Method of restoration; Forensic gemmology; Introduction, Background, Identification.  

Unit-3
Teaching Hours:15
Forensic Photography
 

Basic principles of Photography, Techniques of black & white and color photography, cameras, lenses, shutters, depth of field, film; exposing, development and printing techniques; Different kinds of developers and fixers; UV, IR, fluorescence illumination guided photography; Modern development in photography- digital photography, working and basic principles of digital photography; Surveillance photography. Videography of Crime Scene, laboratory photography. 

Unit-4
Teaching Hours:15
Crime Scene Management
 

Crime scene investigation, types of crime scene; Indoor, Outdoor, Mobile. protection and barricading of crime scene; Documentation, sketching, field notes and photography. Search of evidences; Different types of evidence; Physical vs. non-physical; Real vs. Demonstrative; Known vs. Unknown; Individual vs. Class, handling, collection, preservation and transportation of evidences. Duties of first responder at crime scene, Chain of custody, Reconstruction of scene of crime. Report writing. 

Text Books And Reference Books:

1. Houck, M.M & Siegel, J.A; Fundamentals of Forensic Science, Acadamic Press, London, 2006.

2. Sharma, B.R; Forensic Science in Criminal Investigation & Trials, Universal Publishing Co., New Delhi, 2003

3. James, S.H and Nordby, J.J; Forensic Science- An Introduction to Scientific and Investigative Techniques, CRC Press, USA, 2003.

4. Saferstein; Criminalistics- An Introduction of Forensic Science, Prentice Hall Inc, USA,2007. 

Essential Reading / Recommended Reading

5. H.L. Blitzer and J.Jacobia; Forensic Digital Imaging and Photography, Academic Press, London, 2002.

6. Saferstein Richard, Criminalistics: An Introduction to Forensic Science (11th edition), Pearson, 2015.

7. Houck M. Max & Siegel A. Jay, Fundamentals of Forensic Science (3rd edition), Academic Press, 2015.

8. Kumar K., Forensic Ballistics in Criminal Justice (1st edition), Eastern Book Company, 2015.

9. Ramachandran R., Scientific techniques in criminal investigation (2nd edition), Kamal Publishers, 2021.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS132 - CRIMINOLOGY, CRIMINAL LAW AND POLICE ADMINISTRATION (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to introduce the concept and scope of crime. It will familiarize students with types of crime and its effects as well its prevention. The course would highlight about criminal behavior and related theories. The course aims to discuss the concept of Juvenile delinquency and Victimology. It will disseminate information to students with specific criminal Law, Indian Penal Code: sections, Criminal Procedure Code and police Administration. 

Course Outcome

CO1: To understand concept of crime and recent development in its control and prevention

CO2: To study the aim and scope of criminology.

CO3: To elucidate Criminal profiling and modus operandi, portrait parley, voice stress analysis

CO4: To describe History and development of police administration; Police duties, responsibilities and powers.

CO5: To understand about the different IPC, CrPC sections and different Acts

Unit-1
Teaching Hours:15
Crime and criminal
 

Definition, concept and scope of crime. Concept of criminal, Types of crime. Factors in crime, crime trends among youth, genesis and topology of youth crime, terrorism, sexual offences, effects, control and prevention of crime. latest developments in prevention of crime. Recidivism, Banishment, Dark figures of Crime (Hidden crimes) and Victimization Survey, latest developments in prevention of crime.  

Unit-2
Teaching Hours:15
Criminology and theories of criminal behavior
 

Aim and scope of criminology, Criminology as a science, Criminal behavior and theories of criminal behavior: classic, positivist, sociological, biological, physiological sociological. Organized crimes, white collar crime. crime against women, Juvenile delinquency. Role of correctional institutions. Criminal profiling and modus operandi, portrait parley, Victimology and Penology.  

Unit-3
Teaching Hours:15
Criminal Law
 

Criminal Procedure Code, bailable/non-bailable offences, cognizable/ non-cognizable, summon case and warrant cases. Expert testimony. Indian Penal Code: sections-23, 24, 25,39,40, 44,52,76-79,84-86. 120, 357, 364,377, 391, 395, 399, 400, 402. Applicable sections-2, 6-8, 12, 21, 28, 33, 54, 71. 82, 88. 166, 180. 299, 300, 301, 302,303,304,306, 307,312,313, 314, 315, 316, 319, 320, 354 A, 354 D, 359, 363, 370, 375, 376, 377, Indian Evidence Act and sections- 3, 24-30, 44, 45, 46, 47, 135-138, 141. Information technology act, NDPS Act, Food and Adulteration Act, Drugs and Cosmetic Act, Arms and Explosives Act. 

Unit-4
Teaching Hours:15
Police Administration
 

History and development of police administration; Police duties, responsibilities and powers. Organization and structure of police; maintenance of crime records and accountability of police to law. People and society. Limitations on powers, Custodial crimes, Police and Human Rights. Dimensions of police accountability in India.Introduction to NIA, CBI, BPR&D and Interpol.  

Text Books And Reference Books:

1. Ramachandran R., Scientific techniques in criminal investigation (2nd edition), Kamal Publishers, 2021.

2. Gross. H; Criminal Investigation- A Practical Textbook for Magistrates, Police Officers, and Lawyers; Universal Law Publishing Co., New Delhi, 2000.

3. Lyman, M.D; Criminal Investigation – The Art &the Science, Prentice Hall, New Jersey, 2002.

4. Jack Fitzgerald Statistics for Criminal Justice and Criminology in Practice and Research, CRC press, 2013.  

Essential Reading / Recommended Reading

5. Swansson,C. R, Chamelin, N.C, &Territ, L; Criminal Investigator, Mc Grawhill, New York, 2000.

6. The Indian Evidence Act (1872), Amendment Act; Universal Law Publishing Co., 2003.

7. The Code of Criminal Procedure (1973) Amendment Act, (2001); Universal Law Publishing Co., 2002.

8. Rattan Lal & Dhiraj Lal; The Indian Penal Code, 28th Ed. Wadhwa& Co. Nagpur, 2002.

9. Walter S. De Keseredy, Contemporary Critical Criminology, Routledge publisher, 2021.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS133 - ANALYTICAL INSTRUMENTS AND TECHNIQUES IN FORENSIC SCIENCE (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to introduce specialized techniques and their application in forensic science. The students will be able to understand the atomic & molecular spectroscopy, physical instrumentation techniques, radiochemical and nuclear techniques, biological and biochemical techniques with their general principles involved as well as their applied aspects.  

Course Outcome

CO1: Learn basic principles of analytical techniques in forensic science

CO2: To learn general principles of biological and biochemical techniques

CO3: Strategically plan analytical techniques to apply to different types of samples and research objectives

CO4: Appreciate various instrumentation techniques and find employment in the industry

Unit-1
Teaching Hours:15
Microscopy Techniques
 

Light Microscopy: Basics (Simple and compound, Magnification, Resolution and parts of microscope), Lens and aberrations, Different light microscopy techniques: Bright Field, Dark Field, Phase Contrast, Polarized Light. Comparison, Stereo-Zoom and Fluorescence microscopy techniques. Electron Microscopy: Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Scanning-Transmission Electron Microscopy (STEM), Scanning Tunnelling Microscopy (STM) and Atomic Force Microscopy (AFM), Applications in Forensics 

Unit-2
Teaching Hours:15
Molecular Biological Techniques
 

Basic Concept: Structure, composition and properties of Proteins and Nucleic acid (DNA and RNA), Restriction Endonucleases, Repeat Sequences (STR and VNTR). Gel Electrophoresis: Agarose, Capillary gel electrophoresis, PAGE and SDS-PAGE, 2D Gel Electrophoresis & Iso-Electric Focusing. Polymerase Chain Reaction (PCR), Hybridization (Northern and Southern), and DNA Fingerprinting (RFLP and AFLP) Antibodies: Structure, Types, Western Blot and ELISA, Flow Cytometry, Applications in Forensics

Unit-3
Teaching Hours:15
Spectroscopy and Spectrometry
 

Conventional sources for UV, visible and infrared rays, sources for shorter wavelength radiations (X-ray tubes), radioactivity, Laser (He, Ne Argon, ion, dye lasers, semiconductor-lasers) a source of radiation, interaction of radiation with matter; reflection, absorption, transmission, fluorescence, phosphorescence Colorimetry and Ultraviolet–visible spectroscopy, Fluorescence Spectrometers/Fluorometers, Infrared Spectroscopy (FTIR), Raman Spectroscopy, Chemiluminescence spectroscopy. Mass Spectrometry (Ionization Types-CI, FAB, EI, ESI and MALDI, Mass analyzers: Quadrupole, Triple quadrupole and TOF), Isotope Ratio Mass Spectrometer, Atomic absorption spectrometry (AAS), Inductively coupled plasma atomic emission spectroscopy (ICP-AES), X-ray based technique (EDX, WDX and XRD), Applications in Forensics  

Unit-4
Teaching Hours:15
Chromatography and Centrifugation
 

Chromatography Basics: Types of Chromatography techniques (Column, TLC and Paper; Solid-Liquid, Liquid-Liquid and Gas-Liquid)), Analytical-Semi Preparative and Preparative. Liquid Chromatography: Stationary phases (Adsorption, Ion Exchange, Size Exclusion, Affinity, RP/HIC, Mixed mode), Quality parameters (Resolution, Asymmetry Factor and HETP), Detectors (UV-Vis and Mass), HPLC and UPLC Gas Chromatography: Basics of Instrumentation, Column Properties, Stationary phases (Polar and Non-Polar), Detector (Flame Ionization Detector). Centrifugation: Basics (Principle, RPM & RCF), Types (Differential, Zone, Rate Zonal and Density gradient), Rotors, Scale (Laboratory and Preparative) Applications in Forensics  

Text Books And Reference Books:

1. Siegel A. Jay & Mirakovits Kathy, Forensic Science the basic (3rd edition), CRC Press, 2015.

2. Stuart H. Barbara, Forensic Analytical Techniques, John Wiley & Sons, 2013.

3. Subrahmanyam, N. &Lal B; A text Book of Optics, S. Chand & Company, New Delhi, 2004.

4. Skoog A. Douglas, Holler James F. & Crouch R. Stanley, Principles of Instrumental Analysis (7th edition), Cengage, 2018

Essential Reading / Recommended Reading

5. Khandpur, R. S; Handbook of Analytical Instruments, Tata McGraw Hill Pub. Co. New Delhi, 2004.

6. Saferstein Richard, Criminalistics: An Introduction to Forensic Science (11th edition), Pearson, 2015

7. David J. Thomas & Lewis M. James, Forensic Odontology Principles and Practice, Mica Haley, 2018. 

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS134 - FUNDAMENTALS OF QUESTIONED DOCUMENT EXAMINATION (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to disseminate the knowledge about various types of forensic documents including genuine, forged and holographic documents. It also aims to discuss the principles of handwriting, its identification, tools needed for forensic document examination & their use in forensic science. The course wild explains the typewritten, printed documents and forgery detection techniques to students. 

Course Outcome

CO1: The paper tends to introduce students to document examination. Also describes classification, variation, nature and problems of document examination.

CO2: To learn writing features, their estimation, general characteristics, individual characteristics, ethnic and gender variability of handwriting, various types of forgeries and their detection.

CO3: To identify typescripts, various types of printing processes and their examination

CO4: To Determine identification of age of documents by examination of signatures, paper, ink etc.

CO5: Application of computerized applications in questioned documents examination.

Unit-1
Teaching Hours:15
Introduction to Document Examination
 

Nature and problems of document examination, classification of questioned documents, Specimen/Admitted writings/type writings, handling, preservation of documents, importance of natural variation and causes of natural variation, Disguise writing, characteristics of disguise writing and its examination, various types of forensic documents- genuine and forged documents, holographic documents, tampered documents, principles of handwriting identification, basic tools needed for Forensic Document Examination & their use in analysis of paper and inks, TLC, LC, Video spectral comparator, ESDA.  

Unit-2
Teaching Hours:15
Handwriting and Signature examination
 

General characteristics of handwriting; class and individual characteristics, Various writing features and their estimation, ethnic and gender variability of handwriting, Forgery, various types of forgeries and their identical characteristics, identification of forger, examination of signatures – characteristics of genuine and forged signatures, identification of writer of anonymous letters, application of Forensic Stylistics/Linguistics in the identification of writer, examination of built-up documents and determination of sequence of strokes. 

Unit-3
Teaching Hours:15
Typewritten and Printed Documents
 

Type writers and their individuality, Identification of typescripts-identification of typist, various types of printing processes, identification of printed matter including printing of security documents and currency notes, identification of electronic typewriters, dot matrix, inkjet and laser jet printers, examination of black and white and color photocopies, scanned copies, fax messages and carbon copies. Indian passport/visas, 

Unit-4
Teaching Hours:15
Forgery Detection
 

Determination of age of documents, paper, ink etc., Examination of alterations, erasures, over writings, additions and obliterations, document analysis for decipherment of obliterated and secret writings, charred documents and indentation marks, examination of seal, rubber and other mechanical impressions, examination of counterfeit currency notes, stamp papers, postal stamps, examination of fake credit cards, e-documents, digital signatures, preliminary examination of documents, various computerized approach in documents examination; Cedar, LDMS  

Text Books And Reference Books:

1. Siegel A. Jay & Mirakovits Kathy, Forensic Science the basic (3rd edition), CRC Press, 2015.

2. Sharma R. B., Firearms in Criminal investigation & Trial (5th edition), Universal Law Publishing, 2017.

3. Houck M. Max & Siegel A. Jay, Fundamentals of Forensic Science (3rd edition), Academic Press, 2015.

4. Lewis A. Jane, Forensic Document Examination: Fundamentals and current trends, Academic Press, 2014.  

Essential Reading / Recommended Reading

5. Harrison, W.R; Suspect Documents Their Scientific Examination, Universal Law Publication, Delhi, 2001.

6. Morris, R.N; Forensic Handwriting Identification, Academy Press, London, 2001.

7. Saferstein Richard, Criminalistics: An Introduction to Forensic Science (11th edition), Pearson, 2015

8. Lerinson, J; Questioned Documents, Academy Press, London, 2001.

9. Katherine, M. K; CDE-Forensic Document Examination-Humana Press, New Jersey, 2007. 

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS135 - RESEARCH METHODOLOGY IN FORENSIC SCIENCES (2023 Batch)

Total Teaching Hours for Semester:30
No of Lecture Hours/Week:2
Max Marks:50
Credits:2

Course Objectives/Course Description

 

This coursewill help to understand the theoretical basis of conducting research and basic concepts, To design research problem, research process, Understanding the importance of research paper, dissertation and projects, To impart knowledge regarding the ethics in research.

Course Outcome

CO1: To understand the basic concepts of research

CO2: To find gaps in the existing research of their interest and conduct the research accordingly

CO3: To have the basic knowledge of statistics and tools for data collection.

CO4: To write a project report as well as research and review paper.

CO5: To have the basic knowledge of journals, electronic databases, and ethics.

Unit-1
Teaching Hours:10
Concepts of Research and Research Formulation
 

Basic concepts of research -Meaning, Objectives, Motivation and Approaches. Types of Research (Descriptive/Analytical, Applied/ Fundamental, Quantitative/Qualitative, Conceptual/ Empirical); Research formulation -Observation and Facts, Prediction and explanation, Induction, Deduction; Defining and formulating the research problem, Selecting the problem and necessity of defining the problem; Literature review -Importance of literature reviewing in defining a problem, Critical literature review, Identifying gap areas from literature review; Hypothesis -Null and alternate hypothesis and testing of hypothesis -Theory, Principle.

Unit-2
Teaching Hours:7
Research Design and tools of data collection
 

Research Design -Basic principles, Meaning, Need and features of good design, Important concepts; Types of research designs; Development of a research plan -Exploration, Description, Diagnosis, Experimentation, determining experimental and sample designs, Research process, tools of Data collection; interview, schedule, survey, Questionnaire, Case-Control Studies, Cohort Studies. Scaling process; nominal, ordinal, interval, ratio, significance of scaling in research, Statistics; importance, significance. Measures of central tendency; mean, median, mode. Measures of dispersion; mean deviation, Variance standard deviation. Correlation and types of correlation; Karl Pearson, rank correlation,  

 
Unit-3
Teaching Hours:4
Scientific Documentation and Communication
 

Workbook maintenance, Project proposal writing, Research report writing (Thesis and dissertations, Research articles, Oral communications); Presentation techniques - Assignment, Seminar, Debate, Workshop, Colloquium, Conference.

Unit-4
Teaching Hours:9
Information Science, Extension and Ethics
 

Sources of Information -Primary and secondary sources; Library - books, Journals: Indexing journals, abstracting journals, research journals, review journals, e-journals. Impact factor of journals, NCBI-Pub Med.; periodicals, reference sources, abstracting and indexing sources, Reviews, Treatise, Monographs, Patents. Internet -Search engines and software, Online libraries, e-Books, e-Encyclopedia, TED Talk, Institutional Websites; Intellectual Property Rights - Copy right, Designs, Patents, Trademarks, plagiarism, Geographical indications; Safety and precaution - ISO standards for safety, Lab protocols, Lab animal use, care and welfare, animal houses, radiation hazards; Extension: Lab to Field, Extension communication, Extension tools; Bioethics: Laws in India, Working with man and animals, Consent, Animal Ethical Committees and Constitution.

Text Books And Reference Books:

1.       Thomas, C.G., Research Methodology and Scientific Writing. Anne Books Pvt. Ltd. Bengaluru. 2017.

2.       Dawson, C. Practical research methods. UBS Publishers, New Delhi. 2002.

Essential Reading / Recommended Reading

1.       BUCH, M. B., A Survey of Research in Education, Baroda: CASE, M. S. University, 618 pp. 2014.

2.       Ruzin, S.E. Plant micro technique and microscopy. Oxford University Press, New York, U.S.A., 2009.

3.       Best, John, W.& Kahn, James. Research in Education, 5th ed., Prentice–Hall of India Pvt Ltd: New Delhi. 2006

Evaluation Pattern

Evaluation will be based on 10% CIA 1, 25% CIA 2, 10% CIA 3 and 5% Attendance

MFS151 - PATTERN EVIDENCE AT SCENE OF CRIME, FORENSIC PHOTOGRAPHY AND FORENSIC DOCUMENT EXAMINATION LAB (2023 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This course will help toDeveloping an understanding and application of Crime Scene Investigation, Develop an understanding on processing of crime scene (searching, sketching and photography of Crime scene (Indoor and Outdoor), Brief description on procedure adopted for collection, preservation and packaging of Physical evidences, Develop an understanding on examination of various types of forensic documents, security documents, collection and preservation of various types of documents.

Course Outcome

CO 1: Analyze Indoor and Outdoor Crime Scene and to Identify the evidences at crime scene

CO 2: Demonstration if crime scene through photography, sketching.

CO 3: Learn importance of barricading, chain of custody, crime scene reconstruction.

CO 4: Illustrate method used for trace evidence collection.

CO 5: Examination of various forensic documents and handwritings

Unit-1
Teaching Hours:50
Crime Scene
 

1. To carry out photography of indoor and outdoor crime scenes

2. Crime scene photographic processing and development in different light sources and using different filters.

3. To carry out digital photography of various forensic evidences.

4. Mock crime scene investigation and write a report on evaluation of crime scene.

5. Collection, handling and preservation of various pieces of evidence (Fingerprints, blood, saliva, fibers, hairs, etc.).

6. Various searching methods of evidence at indoor and outdoor crime scenes.

7. Interpretation of crime scene notes, photos, sketches and reconstruction of crime scene

8. Microscopy of various physical evidences recovered from crime scene

9. Expert testimony in a mock court case scenario.

10. Blood spatter analysis.  

Unit-2
Teaching Hours:50
Forensic Document Examination
 

1. Identification of Class and Individual Characteristics of Handwriting.

2. Study of natural variations and fundamental divergences in handwriting. 

3. Comparison of questioned and admitted handwriting’s samples.

4. Detection of Simulated forgery.

5. Detection of traced forgery.

6. Study of Disguise in handwriting documents.

7. Comparison of Typewritten scripts.

8. Security documents i.e. passport, driving license etc. And Currency note examination.

9. Care, handling, preservation, marking, packing and forwarding of documents

10. Examination of rubber stamp, seal and other mechanical impressions.  

Text Books And Reference Books:

1. Houck, M.M & Siegel, J.A; Fundamentals of Forensic Science, Acadamic Press, London, 2006.

2. Sharma, B.R; Forensic Science in Criminal Investigation & Trials, Universal Publishing Co., New Delhi, 2003

3. James, S.H and Nordby, J.J; Forensic Science- An Introduction to Scientific and Investigative Techniques, CRC Press, USA, 2003.

4. Saferstein; Criminalistics- An Introduction of Forensic Science, Prentice Hall Inc, USA,2007

Essential Reading / Recommended Reading

5. H.L. Blitzer and J.Jacobia; Forensic Digital Imaging and Photography, Academic Press, London, 2002.

6. Saferstein Richard, Criminalistics: An Introduction to Forensic Science (11th edition), Pearson, 2015.

7. Houck M. Max & Siegel A. Jay, Fundamentals of Forensic Science (3rd edition), Academic Press, 2015.

8. Kumar K., Forensic Ballistics in Criminal Justice (1st edition), Eastern Book Company, 2015.

9. Ramachandran R., Scientific techniques in criminal investigation (2nd edition), Kamal Publishers, 2021.

Evaluation Pattern

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination – 100 marks

MFS152 - ANALYTICAL INSTRUMENTS AND TECHNIQUES IN FORENSIC SCIENCE LAB (2023 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:4
Max Marks:100
Credits:8

Course Objectives/Course Description

 

This course will help to developing an understanding and application of spectrophotometric and various microscopes, Develop an understanding on implementation of various chromatographic techniques, Brief description on procedure adopted for scanning and identification of drugs, Develop an understanding on examination of software-based method and various analytical instruments.

Course Outcome

CO1: Implementation of various microscopes

CO2: Identification of dyes/ ink by use of chromatographic techniques

CO3: Identification and study of scheduled drugs.

CO4: Learning of isolation of blood by lymphocytes

CO5: To Illustrate manual and software- based method

Unit-1
Teaching Hours:120
Practicals
 

1. Spectrophotometric/Colorimetric estimation of protein by Lowry’s method

2. Light microscopy and measurement of Diatom specimen by micrometry method

3. Thin Layer Chromatography

4. Ink and Pigment separation

5. Study of Chromatogram (GC/LC) and identification of compounds based on standard match

6. Study and identification of scheduled drug by Mass Spectrometry based method

7. Spectral scanning and identification of a few scheduled drugs

8. Yeast mitochondria counting.

9. Enzyme kinetics of catalase enzyme. 

10. DOT ELISA

11. Primer Design for PCR, manual and software-based method

12. Demonstration of various analytical instruments: Gel Electrophoresis (DNA and Protein), Microscopy (Phase Contrast, Bright Field, Fluorescence), Chromatography (HPLC, LC-MS and GC-MS), AAS and FTIR  

Text Books And Reference Books:

1. Siegel A. Jay & Mirakovits Kathy, Forensic Science the basic (3rd edition), CRC Press, 2015.

2. Stuart H. Barbara, Forensic Analytical Techniques, John Wiley & Sons, 2013.

3. Subrahmanyam, N. &Lal B; A text Book of Optics, S. Chand & Company, New Delhi, 2004.

4. Skoog A. Douglas, Holler James F. & Crouch R. Stanley, Principles of Instrumental Analysis (7th edition), Cengage, 2018 

Essential Reading / Recommended Reading

5. Khandpur, R. S; Handbook of Analytical Instruments, Tata McGraw Hill Pub. Co. New Delhi, 2004.

6. Saferstein Richard, Criminalistics: An Introduction to Forensic Science (11th edition), Pearson, 2015

7. David J. Thomas & Lewis M. James, Forensic Odontology Principles and Practice, Mica Haley, 2018.  

Evaluation Pattern

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination – 100 marks

MFS231 - FORENSIC BIOLOGY AND FORENSIC MEDICINE (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to disseminate the knowledge about forensic examination of various types of biological evidences, morphology of human body. It also aims to discuss the Thanatology, various types of deaths, collection and preservation of viscera. The course will explain about various types of injuries their identification and role to establish the identity of an individual. 

Course Outcome

CO1: To know about various biological evidences and their forensic examination and analysis

CO2: Students will understand about the Microbial Forensics and Wildlife Forensics

CO3: To understand the concept of forensic medicine, death, post-mortem changes and determination of time since death

CO4: To understand the concept of wounds/ injuries and its medico-legal aspects.

CO5: Students will get detailed knowledge about the autopsies.

Unit-1
Teaching Hours:18
Forensic Examination of various biological evidences
 

Composition and functions of Blood, Identification of blood stains: Presumptive tests- Benzidine test, Phenolphthalein test, Leucomalachite test, Tetra-Methyl benzidine test and O-Tolidine, Luminol test. Confirmatory testsHaemochromogen test, Haematin test and Haemin test. Typing of blood antigens from dried stains. Latte’s test, Absorption Elution, Absorption Inhibition, Mixed agglutination. Composition and functions of Seminal stains, Identification of seminal stains; Presumptive Tests-Acid Phosphatase Test, Barberios Test and Florence Crystal Test. Confirmatory Test -Sperm Detection. Composition and functions of Saliva, Identification of saliva stains: Starch iodine test, Radial gel diffusion and examination of buccal epithelial cells. Identification of Urine stains: Physical examination, Odor Test, Urea nitrate crystal test and creatinine test. Identification of vomit stains: Detection of Mucus, Free HCL and Endothelial cells. Identification of faecal stains: microscopic detection of undigested food particles, vegetables material and muscle fibers, Urobilinogen Test. Diatoms and Pollen grains, Collection of insects, preservation and shipments of insects, identification and Forensic Significance. Microorganism in biological warfare. Wild Life Forensics, Morphology and biochemistry of Human and Animal Hair and its microscopic examination.

Unit-2
Teaching Hours:15
Introduction to forensic medicine
 

Definition of forensic medicine, cause, manner and characteristics of death. Thanatology- Stages of death. Suspended animation. Determination of time since death, Modes of death. Signs of death and changes following death. Estimation of PM Interval. Entomological evidence and their collection. Deaths due to Asphyxia, Deaths due to fire, electrocution. Starvation, drowning cases, Various Sexual offences and amendment in IPC. 

Unit-3
Teaching Hours:15
Wounds and its medico-legal Significance
 

Injuries –Classification and Medico-legal Aspects. Mechanical Injuries. Firearm Injuries. Thermal Injuries. Explosion Injuries. Electrical Injuries. Atmospheric Lightning. Radiation Injury. Regional Injuries. Traffic Accidents, Medicolegal cases, registration of cases as MLC, Preparation of Medico-legal cases report, Disposal of death cases (legal and non- legal)  

Unit-4
Teaching Hours:12
Autopsy
 

Objectives of Autopsy, Rules for medico-legal Autopsies, Medico-legal versus Hospital Autopsy, Procedure of Autopsy: laboratory procedure, Second Autopsy, obscure Autopsy, Psychological Autopsy, Concept of Virtual autopsy, Micro-radiology and future, Computed Tomography, Preservation of dead bodies, Handling of highly infected bodies, Autopsy report. 

Text Books And Reference Books:

1. Houck, M.M. & Siegel, JA; “Fundamentals of Forensic Science”, Academic Press, London, 2006.

2. Barry, A.J. Fisher; “Techniques of Crime Scene Investigation”, 7th Ed, CRC Press, NY, 2003.

3. David J. Thomas & Lewis M. James, Forensic Odontology Principles and Practice, Mica Haley, 2018.

4. Butler M. John, Fundamentals of Forensic DNA Typing, Academic Press, 2010.

5. Chadha, PV; “Handbook of Forensic Medicine & Toxicology”, Jaypee Brothers, New Delhi, 2004.

Essential Reading / Recommended Reading

6. Bardale Rajesh, Principles of Forensic Medicine and Toxicology, Jaypee Brothers Medical Publishers, 2011.

7. Jones Richard & James-Payne Jason, Simpson’s Forensic Medicine (14th edition), Taylor & Francis group, 2019.

8. Reddy Narayan S.K., The Synopsis of Forensic Medicine and Toxicology (29th edition), Jaypee Brothers Medical Publishers, 2017.

9. Rivers B. David & Dahlem A. Gregory, The Science of Forensic Entomology, Wiley-Blackwell, 2014. 

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS232 - FORENSIC CHEMISTRY AND TOXICOLOGY (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to disseminate the knowledge about basic concepts of chemistry, concentration of alcohol and petroleum products. This paper tends to describe various types of poisons, identification of pesticides. This paper will help to understand the concept of drugs, NDPS act, collection and forwarding of drugs. Further, it will make the students to understand the concept of fire, analysis of debris, various types of explosives and discussion on case studies related to explosives.  

Course Outcome

CO1: To learn basic concepts of chemistry, concentration of alcohol and petroleum products.

CO2: To have the basic knowledge of forensic toxicology, history and significance.

CO3: This paper tends to describe various types of poisons, identification of pesticides.

CO4: To understand the concept of drugs, NDPS act, collection and forwarding of drugs

CO5: To understand the concept of fire, analysis of debris, various types of explosives and discussion on case studies related to explosives

Unit-1
Teaching Hours:15
Forensic Chemistry
 

Scope & significance of Forensic Chemistry, Types of cases/exhibits received for analysis. Trap Cases: Collection, and Preliminary analysis of evidence in trap cases. Alcoholic Beverages: Types of alcohols, country made liquor, illicit liquor, denatured spirits, Indian made foreign alcoholic and non-alcoholic beverages. Dyes: Scope & Significance of dyes in crime investigation, analysis of ink by TLC and UV visible spectrophotometry. Petroleum products and their adulterations: Chemical composition of various fractions of Petroleum Products, Analysis of petrol, kerosene, diesel. 

Unit-2
Teaching Hours:15
Forensic Toxicology
 

Forensic Toxicology - History and Pioneers, Scope and Significance. Classification of Poisons based on their mode of action, uses and origin. Action of poisons, Poisons –Types, routes of administration, toxicity, sign and symptoms. Factors affecting the effect of poison, management and medico-legal aspects of poisoning cases. Isolation and purification of poisons, Common Poisoning in India: Pesticides: Different types and their formulations, identification of pesticides, Guidelines for collecting forensic evidences in poisoning cases at crime scene. Importance of Post mortem examination in poisoning cases. Sample preparation for the analysis of poisons in body tissues/fluids and analysis by various instrumental techniques.  

Unit-3
Teaching Hours:15
Narcotic Drugs and Psychotropic Substances
 

Scope and significance NDPS drugs in forensic science, Classification and characterization of NDPS drugs, Drug Law Enforcement, Search & Seizure, sampling procedure, Forwarding of sample to FSL, Sample preparation for analysis, Preliminary analysis of drugs, quantitative analysis of drugs, instrumental analysis of drugs from hairs, saliva. Reporting of drug cases, Drug abuse, Drug addiction and its problems.  

Unit-4
Teaching Hours:15
Fire/Arson and Explosives
 

Fire: Introduction to Fire & Arson, origin of fire, Chemistry of Fire, Firefighting operations, preservation of fire scene, collection of evidences, Seat of fire, cause of fire, motives, Analysis of fire debris, Case studies related to fire and Arson. Explosive and Explosion: Scope & significance of explosive analysis in forensic science, Types of explosives, deflagration and detonation, explosive trains, collection, preservation and forwarding of exhibits, preliminary analysis of explosives. Do‟s and Don‟ts. Case studies related to explosives.  

Text Books And Reference Books:

1. Lappas T. Nicholas & Lappas M. Courtney, Forensic Toxicology: Principles and Concepts, Shirley Decker-Lucke, 2016.

2. Siegel A. Jay & Mirakovits Kathy, Forensic Science the basic (3rd edition), CRC Press, 2015.

3. Morrison R.T and Boyd R. N.; “Organic Chemistry”, 6th Edition, Prentice Hall, 2003.

4. “Laboratory Procedure Manual: Petroleum Products”, Directorate of Forensic Science, MHA, Govt. of India, 2005.

5. “Working Procedure Manual on Chemistry”, Directorate of Forensic Science MHA Govt. of India, 2005. 

Essential Reading / Recommended Reading

6. Houck M. Max, Forensic Biology: Advanced Forensic Science Series (1st edition), Academic press, 2015.

7. Ramachandran R., Scientific techniques in criminal investigation (2nd edition), Kamal Publishers, 2021.

8. “Laboratory Procedure Manual: Forensic Toxicology”, Directorate of Forensic Science, MHA, Govt. of India, 2005.

9. Stuart H. Barbara, Forensic Analytical Techniques, John Wiley & Sons, 2013.

10. Lewis A. Jane, Forensic Document Examination: Fundamentals and current trends, Academic Press, 2014.

11. “Working Procedure Manual- Chemistry, Explosives and Narcotics”, BPR&D, 2000.

12.. Bardale Rajesh, Principles of Forensic Medicine and Toxicology, Jaypee Brothers Medical Publishers, 2011.

13. Chadha, PV; “Handbook of Forensic Medicine & Toxicology”, Jaypee Brothers, New Delhi, 2004.

14. Modi, JP; “Textbook of Medical Jurisprudence & Toxicology”, N.M. Tripathi Pub, 2001. 

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS233 - FORENSIC BALLISTICS AND FORENSIC PHYSICS (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to disseminate the knowledge about forensic examination of fired cartridge cases, mechanism and very types of ammunition. It also aims to discuss the forensic examination of cartridges cases and gunshot reside. The course will explain about forensic examination of various physical evidences along with speaker identification, recognition and various sources of audio/ video, digital evidences.

Course Outcome

CO 1: To learn basic concepts of forensic ballistics mechanism and types of firearms

CO 2: This paper tends to describe various examination of gunshot residue and cartridge cases.

CO 3: To understand the concept of forensic examination of various types of physical evidences.

CO 4: To understand the concept of sound production, speaker identification and various types of audio-video evidences.

CO 5: To understand the different physical evidence analysis.

Unit-1
Teaching Hours:15
Introduction to forensic ballistics
 

History and development of firearms- their classification and characteristics, various components of small arms, smooth bore and rifled firearms, bore and caliber, shotgun barrels, chokes - their degrees and types; different automatic mechanisms used in small arms – blow back, recoil operated and gas operated mechanisms, rifling, class characteristics of rifled bore, purpose of rifling, methods to produce rifling; trigger and firing mechanism, trigger pull, accidental discharge of firearms, country-made firearms, improvised and imitation firearms. Types of ammunition, nomenclature, percussion caps and their types, various priming composition, propellants, types of cartridge cases, their heads, various types of bullets and their compositional aspects. Safety aspects about handling of firearms and ammunition. 

Unit-2
Teaching Hours:15
Forensic examination of Cartridge case & gunshot residue
 

Physical evidence available in crime involving firearms, principles and practice of identification of firearms, class and individual characteristics, various marks on fired cartridge cases and bullets, test firings, techniques of obtaining test materials, comparison microscope and matching of marks on evidence and test exhibits, automated bullet-cartridge identification system – IBIS and NIBIN. Estimation of range of firing: burning, blackening, tattooing, spread of pellets, Walker‟s test. Chemical tests of copper and lead around gunshot holes. Gun-Shot Residue: Gun-Shot Injuries – caused by shotguns, rifles, revolvers, pistols, evaluation of gunshot injuries. Dermal nitrate test, why was it abandoned, mechanism of formation of gunshot residue, various methods of lifting of gunshot residue, griess reagent test, detection of GSR by AAS. Knowledge of Arms Act. 

Unit-3
Teaching Hours:15
Examination of various physical examination
 

Formation and types of soil, composition of soil, particle size distribution, microscopic examination, density gradient analysis, interpretation of soil evidence, Types of glass and their composition, Forensic examination of glass fractures, determination of direction of impact: cone fracture, rib marks, hackle marks, color and fluorescence, physical matching, refractive index, and interpretation of glass evidence. Types of paint and their composition, macroscopic and microscopic studies, pigment distribution, micro-chemical analysis, solubility test, density and elemental analysis of paint, pyrolysis chromatographic techniques, IR spectroscopy and X-ray diffraction. 

Unit-4
Teaching Hours:15
Speaker recognition, identification and examination of evidences
 

Physics of sound: waves and sound, analysis and synthesis of complex waves, Human and non-human utterances. Vocal anatomy, Speech signal processing and pattern recognition – basic factors of sound in speech, acoustic characteristics of speech signal, Vocal Formants, Forensic Voice Identification: analysis and recording of voice samples in trap/sting investigation., Fourier analysis, analysis of audio-video signal for authenticity, Introduction to the techniques of pattern recognition and comparison. Sound Spectrograph, Voice Spectrographic analysis, Computerized Approach

Text Books And Reference Books:

1. Sharma, B.R.; “Firearms in Criminal Investigation & Trials”, Universal Law Publishing Co Pvt Ltd, New Delhi, 4th Edition, 2011.

2. Saferstein Richard, Criminalistics: An Introduction to Forensic Science (11th edition), Pearson, 2015

3.Houck M. Max & Siegel A. Jay, Fundamentals of Forensic Science (3rd edition), Academic Press, 2015.

4. Skoog A. Douglas, Holler James F. & Crouch R. Stanley, Principles of Instrumental Analysis (7th edition), Cengage, 2018

Essential Reading / Recommended Reading

5.Warlow Tom; Firearms, the Law, and Forensic Ballistics (3rd edition), Taylor & Francis group, 2012.

6. Kumar K., Forensic Ballistics in Criminal Justice (1st edition), Eastern Book Company, 2015.

7.Sharma R. B., Firearms in Criminal investigation & Trial (5th edition), Universal Law Publishing, 2017.

8. Fifield, F.W. and Kealy, D.; “Principles and practice of Analytical Chemistry”, 5th Edition, Blackwell Science, 2000.

9. Christian, G.D.; “Analytical Chemistry”, 6th Edition., John Wiley, 2004

10. Kealey, D. and Haines, P.J.; “Analytical Chemistry”, Bios Scientific/ Viva Books, 2002.  

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS234 - INFORMATION SECURITY AND CYBER CRIME (2023 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to disseminate the knowledge about introduction of cyber-crime and various types of offenses. It also aims to discuss the computer and networking. The course will explain about basics of information security, digital forensic and their examination.

Course Outcome

CO1: To learn basic concepts of cyber -crime and various types of offenses.

CO2: This paper tends to describe the computer and networking.

CO3: The course will explain about basics of information security, digital forensic and their examination.

CO4: To understand the concept of digital forensics data acquisition and evidences.

CO5: Application of various techniques for information security

Unit-1
Teaching Hours:15
Introduction to Cyber Crime
 

Cyber Crime- Overview, Internal and External Attacks, Online and offline attacks. Cybercrimes against Individuals – E-mail spoofing and other online frauds, Phishing and its forms, Spamming, Cyber defamation, Cyberstalking and harassment, Computer Sabotage, Pornographic offenses, Password Sniffing. Cybercrime against organization – Unauthorized access of computer, Denial-of-service (DOS) attack, Distributed Denial of Service (DDoS) attack, Backdoors and Malwares (virus, Trojan horse, worms), E-mail Bombing, Salami Attack, Software Piracy, Industrial Espionage. Cyber Security Policy, Security policies violations, Crimes related to Social Media, ATM, phishing/vishing frauds, Online and Banking Frauds. Intellectual Property Frauds. Cyber Crimes against Women and Children, Phases of cyber-attack.  

Unit-2
Teaching Hours:15
Introduction to Computers and Networking
 

Introduction to Computer Hardware - Various Components of a Computer, Micro-Processor, Memory, Data Storage Devices and Networking components. Understanding Computer Operating Systems (OS), Booting process of computers. Introduction to File Systems and its types. Networking- Digital and Analog Signaling Methods, Network Types and Topologies, Different types of IP Addresses, Network Hardware Devices and Client/Server Computing. 

Unit-3
Teaching Hours:15
Basics of Information Security
 

Information Security - Overview of Information security, CIA Triad, Threats and Vulnerabilities and Risk, Policy, Standards, Procedures, Guidelines and Baselines. Information Asset Classification: Classification of Information, Information Assets – Owner, Custodian, User. Access control, Authentication and Authorization. Information assurance and defensive measures. Digital Document Security. Cryptography: Definitions and Concepts, Symmetric and Asymmetric Cryptosystems, Classical Encryption Techniques – Substitution Techniques, Transposition Techniques, Block Ciphers and Stream Ciphers, Hybrid Encryption Techniques, One-Time Pad. E-mail security, Internet and Web Security. Steganography and Steganalysis.

Unit-4
Teaching Hours:15
Introduction to Digital Forensics
 

Digital Forensics- Introduction, Objective and Methodology, Rules of Digital Forensics, First responder - role, toolkit, do‟s & don‟ts; Search and Seizure of Volatile and Non-volatile Digital Evidence. Imaging and Hashing Digital Evidence, Introduction to deleted file Recovery; Overview of types of Computer Forensics – Network Forensics, Mobile Forensics, Social Media Forensics and E-mail Forensics. Seizing and preserving mobile devices. Methods of acquisition of evidence from mobile devices. Data Acquisition and Evidence Gathering from social media. Introduction to IT Act.  

Text Books And Reference Books:

1. Nina Godbole and Sunit Belapore; “Cyber Security: Understanding Cyber Crimes, Computer Forensics and Legal Perspectives”, Wiley Publications, 2011.

2. Shon Harris, “All in One CISSP, Exam Guide Sixth Edition”, McGraw Hill, 2013.

3. Bill Nelson, Amelia Phillips and Christopher Stuart; “Guide to Computer Forensics and Investigations” – 3rd Edition, Cengage, 2010 BBS.

4. William Stallings; “Cryptography and Network Security: Principles and Practices”, Fifth Edition, Prentice Hall Publication Inc., 2007.  

Essential Reading / Recommended Reading

5. Atul Jain; “Cyber Crime: Issues, Threats and Management”, 2004.

6. Majid Yar; “Cybercrime and Society”, Sage Publications, 2006.

7. Michael E Whiteman and Herbert J Mattord; “Principles of Information Security”, Vikas Publishing House, New Delhi, 2003.

8. Matt Bishop, “Computer Security Art and Science”, Pearson/PHI, 2002.

9. Peter Singer and Allan Fredman; “Cybersecurity and Cyberwar: What Everyone needs to know”, Oxford University Press, 2014. 10. Raef Meeuwisse; “Cybersecurity for Beginners”, Icutrain Ltd., 2015.  

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS235 - ESSENTIALS OF MATHEMATICS AND STATISTICS IN FORENSIC SCIENCE (2023 Batch)

Total Teaching Hours for Semester:45
No of Lecture Hours/Week:3
Max Marks:100
Credits:3

Course Objectives/Course Description

 

Introduction to Statistics for Forensic Scientists is an essential introduction to the subject, guiding the students through the key statistical techniques used to evaluate various types of forensic evidence. With a brief overview of the history and use of statistics within forensic science, the students will be introduced to the different statistical techniques commonly used to examine data obtained during laboratory experiments. There is a strong emphasis on the evaluation of scientific observation as evidence and modern Bayesian approaches to interpreting forensic data for the courts.

Course Outcome

CO1: Students will be able to deliver a critical analysis of the use of statistical tools in the forensic context in a clear, coherent, and understandable way.

CO2: Students will be able to apply basic statistical and probabilistic methods and techniques to analyze the forensic case formulations.

CO3: Select an appropriate test for comparing two populations on a continuous measure when the two-sample t-test is not appropriate.

CO4: Understand and interpret results from Analysis of Variance (ANOVA), a technique used to compare means amongst more than two independent populations.

Unit-1
Teaching Hours:7
Introduction
 

The scope of statistics in forensic science; Classification of study design, Observational studies and Experimental studies (uncontrolled studies, trials with external controls, crossover studies, trials with self-controls, trials with independent concurrent controls); Exploration and presentation of data: Scales of measurement, Tables, Graphs, Histograms, Box and Whisker plots, Frequency polygon, Scatter Plots, Principle component analysis.

Unit-2
Teaching Hours:15
Probability:
 

Definition, mutually exclusive events and addition rule, independent events and multiplication rule. Sampling: Reasons for sampling, methods of sampling, SRS, Systematic, Stratified, Cluster, NPS. Probability distribution: Binomial, Poisson, Gaussian, Standard normal distribution. Drawing inferences from data: Tests of significance: Statistical inference – estimation - testing of hypothesis - t-test, Chi square test (goodness of fit, independence or association, detection of linkages), Z-test, Confidence intervals, Confidence limits, Hypothesis tests, Types of errors, P-values.

Unit-3
Teaching Hours:15
Estimating and comparing means:
 

Decision about single mean (normal population and non-normal population), decision about single group, decision about paired groups, decision about two independent groups, equality of population variances, computer-aided illustration for comparison of means; Comparing three or more means: ANOVA – one way, two-way, A-priori comparison, Posterior or Post Hoc comparison. Statistical methods for multiple variables: Multiple regression, predicting with more than 1 variable, Statistical test for regression coefficient, Role of R and R2 in multiple regression, Confounding variable (ANACOVA), predicting categorical outcomes – logistic regression, discriminant analysis.

Unit-4
Teaching Hours:8
Correlation and Regression:
 

Pearson’s correlation coefficient, Spearman’s rho, Linear regression, Least Square method, predicting with regression equation, comparing two regression lines, dealing with nonlinear observation, Common errors in regression, Comparing correlation and regression.

Text Books And Reference Books:

1. V. B. Rastogi, Biostatistics, New Delhi: Medtec, Scientific International, Pvt. Ltd., 2015. 

Essential Reading / Recommended Reading

2. W. W. Daniel and C. L. Cross, USA: Biostatistics: A Foundation for Analysis in the Health Sciences, 10th ed. John Wiley & Sons Inc., 2012.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: 5 x 20 marks = 100 marks. Out of five, three questions will have choices.

MFS251 - FORENSIC BIOLOGY, FORENSIC CHEMISTRY, AND TOXICOLOGY LAB (2023 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This course will help to developing an understanding of biological evidences and their analysis, Examination of human hairs and fibers and vegetable fibers, Identification of common poisons and their microscopic examination, to test various drugs, preliminary examination and analysis of arson cases.  

Course Outcome

CO1: To identify blood stains, seminal stains, urine and saliva and their examination.

CO2: Identification of human hairs and vegetable fibers, microscopic examination.

CO3: These experiments will help of identify common poisons, color and microscopic examination.

CO4: To understand explosives, various types of drugs, color tests.

CO5: Identify and analyze inorganic poisons by color test and microscopic examination.

Unit-1
Teaching Hours:120
Forensic Biology, Forensic Chemistry, and Toxicology
 

1. Identification of blood stains using enzymatic and crystal tests

2. Identification of seminal stains using presumptive test, crystal test and detection of spermatozoa

3. Identification of saliva stains, preliminary examination and confirmatory examination.

4. Identification of urine stains, color test.

5. Microscopic Examination of Human and Animal Hairs

6. Microscopic Examination of Vegetable Fibers, solubility test and chemical tests.

7. Examination of fibers for elemental analysis (Protein, Sulphur, Nitrogen, Chlorine)

8. Identification of commonly encountered inorganic poisons Arsenic, Antimony, Bismuth, Mercury by color test and microscopic examination.

9. Identification of ethyl alcohol and methyl alcohol by color tests and microscopic examination.

10. Analysis of accelerants and incendiary in Arson cases by TLC and UV visible spectrophotometry.

11. Identification of explosives by color tests & group analysis.

12. Identification of NDPS drugs by color tests and TLC.

13. Extracting poisons from vomit and urine samples.

14. Lab testing of Aluminum Phosphide (Phosphine gas)

15. Color/spot and TLC separation for common drugs of abuse.

16. Analysis of phenolphthalein in trap cases.

17. Identification tests for volatiles (Acetone/ Acetaldehyde/ Ethyl alcohol/)

18. Identification of Basic drugs by colour and screening tests 

Text Books And Reference Books:

1. Houck, M.M. & Siegel, JA; “Fundamentals of Forensic Science”, Academic Press, London, 2006.

2. Barry, A.J. Fisher; “Techniques of Crime Scene Investigation”, 7th Ed, CRC Press, NY, 2003.

3. David J. Thomas & Lewis M. James, Forensic Odontology Principles and Practice, Mica Haley, 2018.

4. Butler M. John, Fundamentals of Forensic DNA Typing, Academic Press, 2010.

5. Chadha, PV; “Handbook of Forensic Medicine & Toxicology”, Jaypee Brothers, New Delhi, 2004. 

Essential Reading / Recommended Reading

6. Bardale Rajesh, Principles of Forensic Medicine and Toxicology, Jaypee Brothers Medical Publishers, 2011.

7. Jones Richard & James-Payne Jason, Simpson’s Forensic Medicine (14th edition), Taylor & Francis group, 2019.

8. Reddy Narayan S.K., The Synopsis of Forensic Medicine and Toxicology (29th edition), Jaypee Brothers Medical Publishers, 2017.

9. Rivers B. David & Dahlem A. Gregory, The Science of Forensic Entomology, Wiley-Blackwell, 2014. 

10. Sharma R. B., Firearms in Criminal investigation & Trial (5th edition), Universal Law Publishing, 2017. 

Evaluation Pattern

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination – 100 marks

MFS252 - FORENSIC BALLISTICS, FORENSIC PHYSICS AND INFORMATION SECURITY LAB (2023 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This course will help to developing an understanding of examination of fired cartridge cases, examination physical properties of various evidences, and microscopic examination, Identification of fibers, preliminary and chemical examination, To compare cloth pieces and reconstruction of broken bangle piece. 

Course Outcome

CO1: To identify and understand of examination of fired cartridge cases.

CO2: Examination physical properties of various evidences, and microscopic examination.

CO3: To understand the pre and post blast residue analysis.

CO4: To apply Information Security in various perspectives in networks.

Unit-1
Teaching Hours:120
Introduction to forensic ballistics
 

1. Examination of comparison of class and individual characteristics of fired bullets.

2. Examination and comparison of fired cartridge cases (caliber, firing pin marks, breech face marks, chamber marks, extractor and ejector marks)

3. Chemical tests for powder residue – Walker’s Test & barrel wash

4. Examination of physical properties of soil and microscopic examination.

5. Density gradient and particle size distribution and pH measurement of soil.

6. Examination of physical properties of glass, physical examination of glass.

7. Color and fluorescence examination of glass, Determination of refractive index of glass,

8. Examination of physical properties of paint, macroscopic and microscopic studies,

9. Micro-chemical analysis, solubility test, IR spectroscopy

10. Physical examination of fiber, microscopic examination, burning tests, solubility test and chemical examination

11. Comparison of different cloth pieces.

12. Analysis and comparison of broken bangles piece.

13. Comparison of Tool marks.

14. Physical matching and glass fracture determination.

15. Microscopic examination of pre-blast and post-blast residue.

16. Nitrate and Nitrite chemical test in explosive materials.

17. Comparison microscope.

18. Heavy element analysis in explosive material by AAS. 

Unit-1
Teaching Hours:120
Introduction to Cyber Information
 

19. IP address finding.

20. Information gathering and searching approaches (system and web

21. Understanding and using networking commands

22. Identifying and removing viruses in the Windows environment

23. Analyzing the data packets based on password sniffing

24. Creating a network design with IP addresses

25. Implementing and testing client-server computing.

26. Creating and integrating nodes in diverse network topologies

27. Managing networking devices for authentication and authorization configuration

28. Securing the document using the digital certificate

29. Classical encryption techniques for network security

30. Reading and analyzing email headers for cyber forensics 

Text Books And Reference Books:

1. Sharma, B.R.; “Firearms in Criminal Investigation & Trials”, Universal Law Publishing Co Pvt Ltd, New Delhi, 4th Edition, 2011.

2. Saferstein Richard, Criminalistics: An Introduction to Forensic Science (11th edition), Pearson, 2015

3.Houck M. Max & Siegel A. Jay, Fundamentals of Forensic Science (3rd edition), Academic Press, 2015.

4. Skoog A. Douglas, Holler James F. & Crouch R. Stanley, Principles of Instrumental Analysis (7th edition), Cengage, 2018 

Essential Reading / Recommended Reading

5.Warlow Tom; Firearms, the Law, and Forensic Ballistics (3rd edition), Taylor & Francis group, 2012.

6. Kumar K., Forensic Ballistics in Criminal Justice (1st edition), Eastern Book Company, 2015.

7.Sharma R. B., Firearms in Criminal investigation & Trial (5th edition), Universal Law Publishing, 2017.

8. Fifield, F.W. and Kealy, D.; “Principles and practice of Analytical Chemistry”, 5th Edition, Blackwell Science, 2000.

9. Christian, G.D.; “Analytical Chemistry”, 6th Edition., John Wiley, 2004

10. Kealey, D. and Haines, P.J.; “Analytical Chemistry”, Bios Scientific/ Viva Books, 2002. 

Evaluation Pattern

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination – 100 marks

MFS331 - FORENSIC ANTHROPOLOGY (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Theobjectiveofthiscourseistoexplaintherelevanceofpersonalidentificationanditsapplicationusingsomatometricandsomatoscopicobservation,tattoomarks,deformitiesandgenetictraitsinforensicinvestigations.Itwillalsoexplainthebasicprinciplesofhumangrowthanddevelopmentwithfocusonmethodsofassessingage-especiallydentalageandskeletalage.Thecoursewouldhighlighttheforensicmorphometryofskeletalremains,skeletalanalysisanddetailsofforensicodontology.

Course Outcome

CO1: To understand genesis and development of forensic anthropology.

CO2: To discuss major stages of human growth and development and different methods of assessing age.

CO3: To describe techniques for recovering skeletonized human remains and their laboratory analysis.

CO4: To learn methods of age estimation from odontological evidences.

CO5: To understand the different methods of facial reconstruction and different indices in Forensic Anthropology

Unit-1
Teaching Hours:15
Personal Identification
 

Scope Importance and significance of forensic anthropology. Personal identification of living individuals,Importanceandneedforpersonalidentification,Documentsproof,scars,professionalmarks,personalarticles, tattoo marks, Finger printings, dentures, age of victim, sex, race, and height of the victim, nails.Genetic traits of forensic significance: Color blindness, ear lobe, brachydactyly, polydactyly, widow’s peak,frontal eminences, Identification oftherecently dead and decomposed bodies. Determination ofAge, Sex,RaceandStaturefromskeletalremains,skull,Pelvis,andlongbones.Darwin’stubercle,supra-orbitalridges,physiognomic

Unit-2
Teaching Hours:15
Human Growth and Development
 

Major stages of human growth and development- Prenatal growth, Postnatal growth and their characteristics,Factor affecting growth- Genetic and Environmental. Methods of studying;Human Growth, Significance ofage in growth studies Methods of assessing age-chronological age, dental age, skeletal age, secondary sexcharacterageandmorphologicalage.Ageassessment-Decimalagecalculation,agegrouping.Chronologicalanddevelopmentalage- Significanceofgrowthstudiesinforensics.

Unit-3
Teaching Hours:15
Forensic Morphometry of Skeletal Remains
 

Natureandformationofbones,Classificationofhumanbones,IdentificationofHumanandAnimalremains.ForensicFacialReconstruction:TwoDimensionaland3ThreeDimensionalMethods,Bonefragments,Pathologicalchangesinbone.ImportanceofPhotofit/IdentityKitSystemforFacialReconstruction.cranioFacialSuperImpositionTechniques(PhotographicSuperImposition,Video-Superimposition,RoentgenographicSuperimposition).UseofSomatoscopicandCraniometricMethodsinReconstruction.GeneticandCongenitalAnomalies:Causes,Types,IdentificationandtheirForensicSignificance.AnthropologicalIndices-cephalicindex,nasalindex,cranialindex,upperfacialindex.

Unit-4
Teaching Hours:15
Forensic Odontology
 

Introductiontohumandentition,Toothstructureandgrowth.typesandfunctionsofdifferentteeth,Estimation of age and sex determination from odontological evidences. Population difference in size andmorphology. Individualization of tooth pulp. Bite marks and its forensic significance. identification in massdisasters,Photography,liftingandpreservationofbitemarks.Typesofbitemarks;collectionandpreservation,Comparisonandevaluationofbitemarkevidences,forensicimportanceofbitemarks;identification- Casestudies,Medico-legalaspectsof bitemarks.

Text Books And Reference Books:

1.    Singh,I.P.&BhasinM.K;AmanualofbiologicalAnthropology,KamlaRajEnterprises,NewDelhi,2004.

2.  Pickering,R.&BachmanD;TheuseofForensicAnthropology,CRCPress,CostaRica,2009.

3.  Shubhra,G;Introductiontoforensicexamination,SelectiveScientificBooks,NewDelhi,2008

4. Michael,W.Haney,H.A.&FreasL.E;TheForensicAnthropologyLaboratory,CRCPress,2008

Essential Reading / Recommended Reading

1.  DavidJ.Thomas&LewisM.James,ForensicOdontologyPrinciplesandPractice,MicaHaley,2018.

 

2.ButlerM.John,FundamentalsofForensicDNATyping,AcademicPress,2010.

 

3.ReddyNarayanS.K.,The Synopsisof Forensic MedicineandToxicology(29thedition), Jaypee BrothersMedicalPublishers,2017.

4.BurnsRameyKaren,ForensicAnthropologyTrainingManual(3rdedition),Pearson,2013.

 

5.ByersN.Steven,IntroductiontoForensicAnthropology(5thedition),RoutledgeTaylor&Francisgroup,2017.

6. RamachandranR.,Scientifictechniquesincriminalinvestigation(2ndedition),KamalPublishers,2021.11.SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

MFS332 - FORENSIC DERMATOGLYPHICS AND OTHER IMPRESSIONS (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to explain the concept of dermatoglyphics and otherimpression with their forensic implication. It will also familiarize students with the biometrics, technologyinvolved and multibiometric systems. The course also aims to discuss the foot/ footwear/tyre impressions lipprints,earprintsand theirsignificanceinforensicinvestigations

Course Outcome

CO1: To understand the history and development of Dermatoglyphics, different classifications used and Modern methodologies in fingerprinting.

CO2: To understand the basics of biometric evidences.

CO3: To understand the science that deals with identification of individuals based on a person's physical and behavioral attributes.

CO4: To learn methods of taking footprints, their collection and identification of characteristics.

CO5: To analyze lip prints and their forensic significance

Unit-1
Teaching Hours:15
Fingerprints and Palm prints
 

Development of Dermatoglyphics, formation of ridges, pattern types, class and individual characteristics.patternarea.Classificationoffingerprints-Henry’ssystemofclassification,single-digitclassification,ExtensionofHenry’sclassification,filing,searchingandfingerprintbureau.Compositionofsweat,developmentofchance,latent,visibleandplasticprints.Preservationandliftingoffingerprints,Conventionalmethodsofdevelopmentoflatentprints-fluorescentmethods,magneticpowdermethod,fuming method, advancement in chemical method etc. Application of laser and other radiations to developlatent fingerprints, metal deposition method and development of latent prints on skin. Utilization of nano-particlesinenhancementoffingerprints,collectionoffingerprintsfromlivinganddeadperson.Ridgecountingandridgetracing,Comparisonofpalmprintsonthebasisofindividualridgecharacteristics.AutomatedFingerprintIdentificationSystem (AFIS).

Unit-2
Teaching Hours:15
Biometrics
 

Definition,Historical developmentofbiometrics, Biometrics within Forensic science, Characteristicsandoperationofbiometricsystem.Classificationofbiometricsystems–physiologicalandbehavioral.PhysiologicalBiometrics;Fingerprints,palmprints,iris,retina,geometryofhandandface.BehavioralBiometrics; Handwriting, signatures, types of forgery, keystrokes, gait and voice and speaker recognition.Strength and weakness of physiological and behavioral biometrics. Multimodal biometrics. Key biometricprocesses–enrollment,identificationandverification.Positiveandnegativeidentification,MeasuresofBiometrics; Performance measures used in biometric systems – FAR, FRR, GAR,FTA, FTE and ATV.Biometricversustraditionaltechnologies.

Unit-3
Teaching Hours:15
Foot/ Footwear/ Tyre impressions
 

Definition, nature,its types, Gait pattern,Casting of footprints indifferent medium, electrostaticlifting oflatentfootprints.Takingofcontrolsamples.Collectionandtracing,lifting,castingofimpressions/footprints,enhancementoffootwearimpressions,analysisandcomparisonoffootimpressions,moulds,identificationcharacteristics.takingofcontrolsamples,skidmarks,treadmarks,significanceandexamination. different types of tyre marks and tool marks encountered, significance of tyre marks and toolmarks,evaluationanditsexamination.

Unit-4
Teaching Hours:15
Lip prints and their significance
 

Human lip anatomy, Aetiology of lip; environmental factors, genetic factors, Nature, location, collection andevaluationoflipprints,typesoflipprints,cheiloscopy,ForensicSignificance,photography,takingofcontrolsamplesoflipprints,Assessmentoflipmorphology;clinicalassessment,anthropometric,ultrasound,three-dimensionalimages,comparisonoflipprints.Moderntechniquesanddevelopments.

Text Books And Reference Books:

1.   Bridges,B.C;CriminalInvestigation,PracticalFingerprinting,ThumbImpression,HandwritingexpertTestimony,OpinionEvidence.,Univ.BookAgency,Allhabad,2000

2.  Jain,A.K.,Flynn,P.&RossA.A.,HandbookofBiometrics,Springer,NewYork2008.

3.  SafersteinRichard,Criminalistics:AnIntroductiontoForensicScience(11thedition),Pearson,2015

4.  DaluzMosesHillary,FundamentalsofFingerprintAnalysis,Taylor&Francisgroup,2015.

5.  SharmaR.B.,FirearmsinCriminalinvestigation&Trial(5thedition),UniversalLawPublishing,2017.

Essential Reading / Recommended Reading

1.  Johari K. C., Forensic Science Identification of Fingerprints (1st edition), Asian Law House, 2018.7.RamachandranR.,Scientifictechniquesincriminalinvestigation(2ndedition),KamalPublishers,2021.

8.  SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015.

9.  RobinsonM.Edward,CrimescenePhotography(2ndedition),Academicpress,2010.

Evaluation Pattern

 

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: Consist of 1 section. This section will consist of 5 questions carrying 20 marks each out of which students will get an option in any two questions  (20 X 5marks = 100 marks).

MFS333 - FORENSIC GENETICS AND FORENSIC SEROLOGY (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Theobjectiveofthiscourseistoexplaintheconceptofhumangenetics,roleofgenesandmutations.Itwillalsofamiliarizestudentswiththeserologicalexaminationanddeterminationofspecies.Thecoursealsoaimstodiscussthevarioustechniquesusedinbloodgroupexaminationandenzymes.

Course Outcome

CO1: To explain the concept of human genetics, role of genes and mutations.

CO2: It will also familiarize students with the serological examination and determination of species.

CO3: The course also aims to discuss the various techniques used in blood group examination and enzymes.

CO4: To facilitate familiarity with biochemical profiling antibody profiling and persistent disease agents.

CO5: To understand the immunological aspects behind the serological analysis

Unit-1
Teaching Hours:15
Human Genetics
 

Principles of heredity, Mendelian Inheritance; Epistasis, Multiple Allelism, Mutations; Mutagens and theirtypes(Physical,chemical,biological),Heredity,Alleles,Humangenetics,Geneticsofpaternity,Humangenetic variations. Mutations & population Genetic Hardy Weinberg Equilibrium. Mutation- their types andcauses.Relevanceofpopulationgenetics.Allelefrequency,genotypefrequency.Polymorphismandheterozygosity. Measures of genetic variations. Interaction of Genes; Supplementary genes, Complementarygenes,Pleiotropy,Lethalgenes,Mutation:Definitionandtypes,Spontaneousandinducedmutation,ChromosomalaberrationMutation,

Unit-2
Teaching Hours:15
Serological examination
 

Immune system, immune response, innate and acquired immunity, antigens, antibodies, and their reactions.Determination of species of origin-ring test, single diffusion in one dimension and two-dimension, doublediffusioninonedimensionandtwodimensions,immune-electrophoresis,rocketimmune-electrophoresis,two-dimensionalelectrophoresis,cross-overelectrophoresis,Anti-humanglobulinseruminhibitiontest,passive haem-agglutination method, precipitin-inhibition test, mixed agglutination method, sensitized latexparticlemethod.Lectinsandtheirforensicsignificance,methodsofsterilizationemployforserologicalwork.

Unit-3
Teaching Hours:15
Blood group examination
 

Human blood group systems. History, biochemistry and genetics of ABO, Rh, Mn and other forensicallysignificantbloodgroupsystems.MethodsofABObloodgrouping(absorption-inhibition,mixedagglutinationand absorptionelution) from blood stainsand other body fluids/stains. Newapproaches inbloodstain grouping. Blood group specific ABH substances. Secretors and non- secretors. Blood groups thatmake racial distinctions. Lewis antigen. Bombay Blood groups. HLA antigens and HLA typing. Role ofsero-geneticmarkersinindividualizationandpaternitydisputes.Pitfallsinredcelltyping.

Unit-4
Teaching Hours:15
Enzymes
 

Biomarkers of individualization;definition and classification.   Red cell enzymes: Genetics,Polymorphismandtyping of PGM, GLO-I,ESD, EAP, AK, ADA etc. and their forensic significance. Serum proteins:Genetics,polymorphismandtypingof– Hb,HP,TF,Bf,C3 etc.and theirforensicsignificance.Non-geneticapproachestoindividualization-biochemicalprofiling,antibodyprofilingandpersistentdiseaseagents.

Text Books And Reference Books:

1.  Goodwin,William;“AnIntroductiontoForensicGenetics”,JohnWiley&SonsLtd.,2007.


2.  Gupta,S.K;“EssentialsofImmunology”,AryaPublications,2008.

3.  Kothari,ManuL;“EssentialsofHumanGenetics”,UniversityPress(India)Pvt.Ltd.,2009.

4.  SinghB.D.;“FundamentalsofGenetics”,KalyaniPublishers,2006.

5.  SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015.

Essential Reading / Recommended Reading

1.  HouckM.Max,ForensicBiology:AdvancedForensicScienceSeries(1stedition),Academicpress,2015.

 

2.    SkoogA.Douglas,HollerJamesF.&CrouchR.Stanley,PrinciplesofInstrumentalAnalysis(7th

edition),Cengage,2018

 

3.  LiRichard,ForensicBiology(2ndedition),Taylor&Francisgroup,2015.

4.      ElenaPilli,AndreaBertiForensicDNAAnalysisTechnologicalDevelopmentandInnovativeApplications,Routledge TaylorandFrancis,2021.

5.      ButlerM.John,FundamentalsofForensicDNATyping,AcademicPress,2010.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: Consist of 1 section. This section will consist of 5 questions carrying 20 marks each out of which students will get an option in any two questions  (20 X 5marks = 100 marks).

MFS334 - FORENSIC DNA PROFILING AND BIOINFORMATICS (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to explain the concept of structure of DNA & RNA andforensicDNAtyping.Itwillalsofamiliarizestudentswiththecollection,preservationofserologicalsamples, variousDNA extraction methods byusing various advanced methods. The course also aims todiscussthevariousapplicationofDNAinforensicandbioinformaticstechniques.

Course Outcome

CO1: To understand the objective of this course is to explain the concept of structure of DNA & RNA and forensic DNA typing.

CO2: To understand and also familiarize students with the collection, preservation of serological samples, various DNA extraction methods by using various advanced methods.

CO3: To learn various application of DNA in forensic and bioinformatics techniques.

CO4: To learn various statistical evaluation of DNA profiles by use of bioinformatics tools

Unit-1
Teaching Hours:8
DNA -A tool for identification
 

ConceptofHeredity,Alleles,MutationsandGeneticspolymorphism.PopulationgeneticsandHardy-Weinberglaw,Calculationofallelefrequencies.DNAthescientificbasisofDNAtyping,Ashorthistoryand milestones in development of forensic DNA typing, Legal perspective and admissibility of DNA – legalstandardforadmissibilityofDNAprofilingprocedural&ethicalconcerns

Unit-2
Teaching Hours:12
DNA sampling and processing
 

Sources of DNA with respect to forensic application (blood, semen, saliva, urine, faeces, hair, teeth, bone,tissueandcells,touchDNA,microbialDNA),Qualityandquantityofthesamplesandqualityandquantityof DNA, Sample collection, Transportation, and preservation of samples for DNA analysis. DNA ExtractionMethods.QuantificationandQualityassessmentmethods.Agarosegelelectrophoresis(AGE),Polyacrylamide Gel Electrophoresis (PAGE), Capillary gel Electrophoresis- Principle and Instrumentation,ForensicIssues:DegradedDNA,contamination,mixedsamples,andlowcopynumber.Resultinterpretation,Qualityassurance inDNAfingerprinting.

Unit-3
Teaching Hours:20
Techniques in DNA profiling
 

PCR Basics, process, PCR Reaction set up, Gradient and Hot start PCR, multiplex PCR, PCR inhibitors andPCR enhancers, Trouble shooting of PCR, controls in PCR, advantages and disadvantages of PCR, RT-PCR,Real time PCR, Random amplified polymorphic DNA (RAPD), Restriction Fragment Length Polymorphism(RFLP), PCR-RFLP, Amplified Fragment Length Polymorphism (AFLP), Variable Number Tandem Repeat(VNTR),ShortTandemRepeat(STR),SingleNucleotidePolymorphism(SNPS),MarkersonXchromosome and its uses, Markers on Y chromosomes and its uses, mtDNA profiling (HVR1 and HVR2profiling),DNAsequencing(SangersandNGS),mRNAmarkersinbodyfluididentification

Unit-4
Teaching Hours:14
DNA statistics and Bioinformatics
 

EvaluationofDNAprofileresults,frequencyestimatecalculations,interpretations,allelefrequencydetermination,matchprobability,Paternity/Maternityindex,Siblingindex,Probabilityofmatch,anddiscrimination,discriminationpower,powerofexclusion,errorsininterpretation.IntroductiontoBioinformatics,DNAdatabases(GenBank,DDBJ,EMBL,CODIS,NDIS,INTERPOLDNADatabase(IDD), Europol Information System (EIS), Status of National DNA databank in India), The transnationalexchange of DNA data,sequence alignment tools-BLAST, CLUSTAL,BioEdit,Dendrogramandsoftwarefordendrogram

Unit-5
Teaching Hours:6
Case studies with DNA profile
 

EarlyapplicationsofDNAprofiling(ImmigrationcaseofAndrewandserialmurdercaseofColinPitchfork), Identification of individuals of air craft accidents cases, identification of victims from the burnedremnants, Identification of accused in gangrape cases, Forensic investigation into Romanov Mystery, DNAprofilingfrom transplantedbonemarrow.

Text Books And Reference Books:

1.  Rudin,Norah;“AnIntroductiontoForensicDNAAnalysis”,CRCLeviwPublishers,2002.

2.   Singh,Yashpal;“DNATestsinCriminalInvestigationTrial&PaternityDisputes”,AliaLawAgency,2006.

3.  Vij,Krishan;“BasicsofDNAandEvidentiaryIssues”,JaypeeBrothers,2004.

4.  J.M.Butler;“ForensicDNATyping”,ElsevierAcademicPress,2005.

5.   S.C.Rastogi,N.Mendiratta&P.Rastogi;“Bio-informatics–MethodsandApplications”,PHIlearningPvt.Ltd.,2009.

Essential Reading / Recommended Reading

1.  Dr.Westhead,JHParish&R.M.Twyman;“Bio-informatics”,VivaBooksLtd.,2003.

2.   G.AO’Gara,Medgyerietal;“ElectrophoresisintheseparationofBiologicalMacromolecules”,JohnWiley&Sons,2008.


3.  JMClaverieandCedricNotedame;“Bioinformatics–ABeginner’sGuide”,WileyPublications,2008.

4.  JPBaride,A.P.Kulkarni&R.D.Mazumdar;“ManualofBiostatistics”,JaypeePublications,2003.

5.      J.ThomasMcclintock;“ForensicDNAAnalysis”,LewisPublications,2008.

6.       P.S.S Sundar RaoandJ.Richard;“IntroductiontoBiostatisticsand ResearchMethods”, 5thEdition,2012.

7.      ButlerM.John,FundamentalsofForensicDNATyping,AcademicPress,2010.

8.         ElenaPilli,AndreaBertiForensicDNAAnalysisTechnologicalDevelopmentandInnovativeApplications,Routledge TaylorandFrancis,2021.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: Consist of 1 section. This section will consist of 5 questions carrying 20 marks each out of which students will get an option in any two questions  (20 X 5marks = 100 marks).

MFS335 - FUNDAMENTALS OF FORENSIC PSYCHOLOGY (2022 Batch)

Total Teaching Hours for Semester:45
No of Lecture Hours/Week:3
Max Marks:100
Credits:3

Course Objectives/Course Description

 

Theobjectiveofthiscourseistoexplaintheconceptofpsychologyandpsychopathologyinforensicscience.Itwillalsofamiliarizethestudentswithseveralpsychologicalexaminationmethods used for interrogation.The coursealso aims todiscuss the application polygraph andits legal aspectsalong withhuman rights of an individual.Thispaper alsoenlightensnarco-analysisandBOES,legalandethicalaspects.

Course Outcome

CO1: Students will understand the concepts and usage of Forensic Psychology

CO2: Students will learn about the different types of Psychological disorder and mental disorder

CO3: Students will learn the importance of different tests used in Forensic Psychology

CO4: Students will have a direction about the ethical concepts about the Polygraph tests

CO5: To understand the principle, methodology and procedure of Narco analysis and Brain mapping

Unit-1
Teaching Hours:11
Introduction to psychology in forensic science
 

Definitionandfundamentalconceptsofforensicpsychologyandforensicpsychiatry.PsychologicalDisorder,   Psychopathology.   Psychological   assessment   and   its   importance.            Assessmentofmentalcompetency.Mentaldisordersandforensicpsychology.Psychologyofevidence–eyewitnesstestimony,confessionevidence.ForensicPsychologyandtheLaw,EthicalIssuesinForensicPsychology,Civilandcriminalcaseassessment,ForensicPsychologicalevidence,CrimeandPsychopathology,GeneticsandCrime,Serialmurders,ModusOperandi;Serialmurderers.Psychologyofterrorism.Biologicalfactorsandcrime–sociallearningtheories,psycho-socialfactors,Insanity,McNaughton’sRule,DurhamRuleofInsanity

Unit-2
Teaching Hours:12
Psychological Examination
 

Psychological Assessment: Psychological Assessment Tools, Detection of deception, Various methods fordetectionofdeception,Interview,Non-verbaldetection,statementassessment,Hypnosis,,voicestressanalyzer,Polygraph,thermalimaging,BrainElectricalOscillationSignatureProfiling,scientific,basis,methods,operationalenvironment,application,utility,limitationsandlegalstatus.FunctionalMagneticResonance study, Current research in detection of deception/truth finding mechanisms. Psychological tests,typesof tests,PersonalitytestsandThe MMPItest

Unit-3
Teaching Hours:11
Polygraph and legal aspects
 

Polygraph:HistoricalaspectsofPolygraph,Principlesofpolygraph,psychophysiologicalaspects,operationalaspects,Questionformulationtechniquesandoperational,interviewingtechniqueprocedure,The Art-Polygraph, Legal and Ethical aspects,Competence to stand trialand use of psychological tests,Humanrightsofindividual.Neurobiologicalforensictestingandinvestigation.

Unit-4
Teaching Hours:11
Narco analysis, Brain Fingerprinting & BEOS
 

Narco-Analysis: Historical aspects, Principle and Theory, General Procedure –Legal and Ethical aspects,Human rights of individual. Brain fingerprinting Brain Electrical Oscillation Signature (BEOS) Profiling:PrincipleandTheory,GeneralProcedure–LegalandEthicalaspects,Humanrightsofindividual.Forensic assessment & treatment of sexual offenders & their victims. Ethical & legal issues in forensicpractice.

Text Books And Reference Books:

Gordon,N.andFleisherW.Effectiveinterviewing&interrogationtechniques.Thirdedition.ElsevierPublication2010.

Bartol,C.R.andBartol,A.M.IntroductiontoForensicPsychology.Researchandapplication,2Edn,BarnesandNoblesNewYork,2008.

Ciccarelli,S.K.andWhite,J.N.Psychology.PearsonLearningSolutions.Boston,2012.


Bartol,C.R.andBartol,A.M.IntroductiontoForensicPsychology.Researchandapplication,2Edn,BarnesandNoblesNewYork,2008.

 

Essential Reading / Recommended Reading

1.      SafersteinRichard,Criminalistics:AnIntroductiontoForensicScience(11thedition),Pearson,2015.

 

2.      HouckM.Max&SiegelA.Jay,FundamentalsofForensicScience(3rdedition),AcademicPress,2015.

 

3.      Veeraraghavan,V.HandbookofForensicPsychology,SelectScientificPublishers,NewDelhi.2009.

 

4.      Virudhagirinathan,B.S.&Karunanidhi,S.,"CurrentstatusofpsychologyandclinicalpsychologyinIndia-Anappraisal",InternationalReviewofPsychiatry,2014.

 

5.      Patherick,W.,Turvey,B.andFergusson,C.ForensicCriminology,ElsevierAcademicPress.USA.2009

 

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%),

Attendance (5%) and End Semester Examination

( CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks CIA2: MID SEMESTER EXAMINATION for 50 marks CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

 

END SEMSTER EXAMINATION: Consist of 1 section. This section will consist of 5 questions carrying 20 marks each out of which students will get an option in any two questions.  (5x20=100 marks)

MFS351 - FORENSIC ANTHROPOLOGY LAB (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:2

Course Objectives/Course Description

 

This course will help to Determine morphological structure of hair and itsexamination,experiments on Skeltonremnants to study age and gender determination of gender and age from long bones to identify the age and gender from teeth.

Course Outcome

CO1: Students will learn to identify the different types of bones

CO2: Students will able to identify the age based on male and female skull

CO3: Stature estimation can be identified from long bones

CO4: Students can differentiate between male and female on the basis of pelvis

CO5: Students can classify the different types of bite marks

Unit-1
Teaching Hours:60
Practicals of forensic Anthropology and odontology
 

1.   SomatoscopicmeasurementsoflivingIndividualslikehaircolor,eyecolor,facialformnoseshape,lipsshapeetc.

 

2.  Craniometricmeasurementsofskull

 

3.  Identificationofhumanbonesanddeterminationoftheirsides.

4.  Determinationofageandgenderfromskull

5.  Statureestimationfromlongbones.

6.  DeterminationofgenderfromPelvis

7.  Determinationofageandgenderfromteeth.

8.  Identificationofanindividualbasedonmorphologicalfeaturesofteeth.

9.  ComparisonandIdentificationofIndividualsfrombitemarks.

10.  Estimationofagefromtheteeth.

 

11.  Bitemarksidentificationondifferentsurfaces

 

a.       Livingsubject

b.      Maxilla&mandible


12.  Examinationof3DphotographsforBitemarks.

13.  DNAprofilingfromtooth/burnttooth.

Text Books And Reference Books:

 

1. Singh, I.P. &Bhasin M.K; A manual of biological Anthropology, Kamla Raj Enterprises, New Delhi, 2004.

2. Pickering, R. & Bachman D; The use of Forensic Anthropology, CRC Press, Costa Rica, 2009.

3. Shubhra, G; Introduction to forensic examination, Selective Scientific Books, New Delhi, 2008

4. Michael, W. Haney, H.A. &Freas L.E; The Forensic Anthropology Laboratory, CRC Press, 2008.

Essential Reading / Recommended Reading

 

5. David J. Thomas & Lewis M. James, Forensic Odontology Principles and Practice, Mica Haley, 2018.

6.Butler M. John, Fundamentals of Forensic DNA Typing, Academic Press, 2010. 

7.Reddy Narayan S.K., The Synopsis of Forensic Medicine and Toxicology (29th edition), Jaypee Brothers Medical Publishers, 2017. 

8.Burns Ramey Karen, Forensic Anthropology Training Manual (3rd edition), Pearson, 2013.

Evaluation Pattern

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination – 100 marks

MFS352 - FORENSIC SEROLOGY, DNA PROFILING AND BIOINFORMATICS LAB (2022 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Thiscoursewillhelpdevelopinganunderstandingofexaminationvariousbloodgroupingtests,experimentsonelectrophoresisofredcellisozymes,DNAExtractionfrombiologicalsamplesusingadvancedmethods,tounderstandandimplementvariousbioinformaticstools.

Course Outcome

CO1: To identify and develop an understanding of examination various blood grouping tests.

CO2: Experiments on electrophoresis of red cell isozymes

CO3: DNA Extraction from biological samples using advanced methods.

CO4: To learn to understand and implement various bioinformatics tools.

CO5: To understand the blood spatter pattern analysis.

Unit-1
Teaching Hours:60
Forensic Serology
 

1.  Determinationofspeciesoforiginofblood,semenandsaliva.

2.    ABOgroupingofbloodstainsbyabsorptionelution,absorptioninhibitionandmixedagglutinationtechniques.

3.  ABOgroupingfromhairroot

4.  Rhgroupingofbloodstains

5.  MNgroupingofbloodstains

6.  Determinationofsecretorstatusfromsalivabyinhibitiontechniques.

7.  Experimentsonelectrophoresisofredcellisozymesviz.PGM,GLO,EsD,EAP,ADA,AK.

8.  ExperimentsonelectrophoresisofserumproteinsHp,Tf,C3,Bf,Gcetc.

9.  Menstrualbloodanditsexaminationbymicroscopicandelectrophoreticmethods.

10.  Faecalstains:chemicalandmicroscopicalexamination,testingofurineandsweat.

11.  Examinationofsalivaanditsstains:microscopicalandchemicaltests.

12.  Examinationofbloodstains:physicalandchemicaltests;spectroscopicexamination.

13.   Examinationofseminalstains:crystaltests,chemical,biochemical,microscopicalandelectro-immuno-diffusiontest.

Unit-2
Teaching Hours:60
Forensic DNA profiling and Bioinformatics
 

ForensicDNAProfiling

1.    DNAExtractionfrombiologicalsamples(Bloodandotherbodyfluidsandtissues)usingOrganic(Phenol-Chloroform)Method

2.  DNAExtractionfrombiologicalsamplesusingChelaxMethod.

3.  DNAExtractionfrombiologicalsamplesusingSaltingoutMethod.


4.  DNAExtractionfrombiologicalsamplesusingcommerciallyavailablekits

5.  QualitativeandQuantitativeAnalysisusingAgarose,UVSpectrophotometerandRealtime-PCR.

6.  PCRAmplificationofDNAsamples

7.  Amp-RFLPAnalysisofbiologicalsamples

8.  STRtypingusingverticalpoly-acrylamidegelelectrophoresisandsilverstaining.

 

Bioinformatics

1.      Dockingstudiesofligands

2.      Constructionofdendogram

3.      Pubmed,

4.      BLASTN,BLASTPandBLASTX

5.      FASTA

6.      KEGG

Text Books And Reference Books:

1.  Rudin,Norah;“AnIntroductiontoForensicDNAAnalysis”,CRCLeviwPublishers,2002.

2.   Singh,Yashpal;“DNATestsinCriminalInvestigationTrial&PaternityDisputes”,AliaLawAgency,2006.

3.  Vij,Krishan;“BasicsofDNAandEvidentiaryIssues”,JaypeeBrothers,2004.

4.  J.M.Butler;“ForensicDNATyping”,ElsevierAcademicPress,2005.

5.   S.C.Rastogi,N.Mendiratta&P.Rastogi;“Bio-informatics–MethodsandApplications”,PHIlearningPvt.Ltd.,2009.

Essential Reading / Recommended Reading

1.  Dr.Westhead,JHParish&R.M.Twyman;“Bio-informatics”,VivaBooksLtd.,2003.

2.   G.AO’Gara,Medgyerietal;“ElectrophoresisintheseparationofBiologicalMacromolecules”,JohnWiley&Sons,2008.


3.  JMClaverieandCedricNotedame;“Bioinformatics–ABeginner’sGuide”,WileyPublications,2008.

4.  JPBaride,A.P.Kulkarni&R.D.Mazumdar;“ManualofBiostatistics”,JaypeePublications,2003.

5.      J.ThomasMcclintock;“ForensicDNAAnalysis”,LewisPublications,2008.

6.       P.S.S Sundar RaoandJ.Richard;“IntroductiontoBiostatisticsand ResearchMethods”, 5thEdition,2012.

7.      ButlerM.John,FundamentalsofForensicDNATyping,AcademicPress,2010.

8.         ElenaPilli,AndreaBertiForensicDNAAnalysisTechnologicalDevelopmentandInnovativeApplications,Routledge TaylorandFrancis,2021.

Evaluation Pattern

CIA Evaluation Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination – 100 marks

 

MFS441A - FORENSIC DRUGS ANALYSIS (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to explain the concept of introduction of drugs, varioustypesofdrugs.Itwillalsofamiliarizestudentswiththecollection,preservationofvariousformofdrugstheirpreliminaryandinstrumentalanalysis.Thecoursealsoaimstodiscussthevariousapplicationclandestine requirement of its examination and facilities. Thissubject willfocus on an emerging aspect ofdrugsinsports,itsidentification andlegalaspects.

Course Outcome

CO1: To understand the concept of introduction of drugs, various types of drugs

CO2: To understand and familiarize the students with collection, preservation of various form of drugs their preliminary and instrumental analysis.

CO3: To have the basic knowledge of illegal manufacture of controlled substances.

CO4: To learn application of clandestine requirement of its examination and facilities.

CO5: To learn about the emerging aspect of drugs in sports, its identification and legal aspects.

Unit-1
Teaching Hours:15
Introduction of drugs
 

Definition of Drug, Drug Use &Misuse, DrugChemistry, Drug Dependence and chemistry ofAddiction,Drug Receptors and Brain Chemistry. Drugs of Abuse: Definition, Classification based on Form and Origin,Use, Effects and Schedules, Structure of NDPS Act and the definitions of each drug classification, Drugs asEvidence,ProfilingExamplesofIllegalDrugs,UnitedNationsInternationalDrugControlProgramme.

Unit-2
Teaching Hours:15
Examination of Various types of drugs
 

AnalysisofDrugsofAbuse:Origin,Pharmacology,Methodsofpreparation,Storage,DiluentsandAdulterants,SampleHandling,OptimizationofExperimentalConditions,Presumptive/ScreeningandConfirmatoryMethods:Color/spottest,Microscopicexamination,Microcrystallinetests,Thin-LayerChromatography,SamplePreparationbeforeTLCSpecimen,ExtractionEvaluationofTLCforDrugScreening, Immunoassay Methods, UV Spectrophotometry, IR/FTIR Spectrophotometry, NMR, GC-MS &HPLC/LC-MS,LegalImplicationsandDataInterpretationofOpiumandOpioidsanalgesics,Stimulants(Cocaine,Amphetamine&otheramphetaminederivatives),Depressants(BarbituratesandBenzodiazepines), Hallucinogens (Cannabis, LSD, Psilocybine and Mescaline), OTC, Inhalant and VolatileSubstances,Drugsinsexualassault

Unit-3
Teaching Hours:15
Introduction of Clandestine
 

Clandestinelaboratory:MeaningandDefinitionofClandestine,ClandestineLaboratory,LabOperators,Manufacturing Process:Extraction, Conversion,Synthesis, and Tableting,The needs triangle:EquipmentNeeds:Reflux,Distillation,Hydrogenation,BucketChemistry,Extractions,ChemicalNeeds,andknowledgeneeds,HarmsCausedbyClandestineDrugLabs,Sceneprocessing,

Designerdrugs:Definition,AnalogsofFentanylandMeperidine(bothsyntheticopioids),Phencyclidine(PCP),Amphetaminesandmethamphetamines(whichhavehallucinogenicandstimulantproperties).LaboratoryAnalysis:TheChemist,Extractions:PhysicalExtraction,Dry Wash/Extraction,Liquid/LiquidExtractions, Analysis: Chemical Color Tests, Microscopic Techniques, Infrared Spectroscopy, Thin-LayerChromatography, Ultraviolet Spectroscopy, Gas Chromatography. Format of NDPS Report Writing & CourtRoomTestimony.

Unit-4
Teaching Hours:15
Drugs in sports
 

Drug Abuse in Sports: Introduction, International Olympic Committee (IOC), World Anti-Doping Agency(WADA),Nationalanti-dopingagency(NADA),ClassificationofcommonlyprohibitedsubstancesandPerformanceenhancingMethods,Steroids,StackandPyramidmethods,BloodDoping,Genedoping,Masking agents, Analysis of drugs from hair, Urine and other Body Fluids and hair and its harmful effects,Sampling techniques, ethical consideration, analytical approaches, Determination of sex of Athletes/sportspersonnel,Presentationof evidence,legalaspects

Text Books And Reference Books:

1.   Clarke’sAnalysisofDrugsandPoisons,(FormerlyIsolation&IdentificationofDrugs)3rdEd.2Vol.Set.2012

2.   BardaleRajesh,PrinciplesofForensicMedicineandToxicology,JaypeeBrothersMedicalPublishers,2011.

3.    JonesRichard&James-PayneJason,Simpson’sForensicMedicine(14thedition),Taylor&Francisgroup,2019.

4.   ReddyNarayanS.K.,TheSynopsisofForensicMedicineandToxicology(29thedition),JaypeeBrothersMedicalPublishers,2017.

Essential Reading / Recommended Reading

1.  HouckM.Max&SiegelA.Jay,FundamentalsofForensicScience(3rdedition),AcademicPress,2015.6.RiversB.David&DahlemA.Gregory,TheScienceofForensicEntomology,Wiley-Blackwell,2014.7.SkoogA.Douglas,HollerJamesF.&CrouchR.Stanley,PrinciplesofInstrumentalAnalysis(7thedition),Cengage,2018

8.Ramachandran R., Scientific techniques in criminal investigation (2nd edition), Kamal Publishers, 2021.9.HouckM.Max,ForensicBiology:AdvancedForensicScienceSeries(1stedition),Academicpress,2015.9.SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015.

10.LappasT.Nicholas&LappasM.Courtney,ForensicToxicology:PrinciplesandConcepts,ShirleyDecker-Lucke,2016.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%)

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

End semester examination : 100 Marks

MFS441B - FORENSIC BIOLOGY AND WILDLIFE FORENSICS (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to explain the concept of forensic biological evidencesincluding; blood, semen, saliva, sweat and their identification by using emerging technologies. It will alsofamiliarize students’ alcohol toxication their preliminary and instrumental analysis. The course also aims todiscussthevariousapplicationofanimalpoison,identificationandexaminationprocess.

Course Outcome

CO1: Obtain the knowledge about the preliminary and confirmatory examination of biological fluids

CO2: Identify the classification and mode of plant and fungal poisons

CO3: understand how forensic entomology assists in death Investigations

CO4: learn about animals, diatoms, plants and plant parts of forensic significance.

CO5: understand how wildlife Forensics aid in Conserving Natural Resources and learn various environmental acts

Unit-1
Teaching Hours:15
Introduction to biological fluids
 

Compositionofbodyfluids-blood,semen,saliva,vaginalfluid,urine,sweatandmenstrualblood.Identificationofbloodstains,seminalstain,salivastain,vaginalfluid,urine,sweatandmenstrualbloodusingcurrentandemergingtechniques.Distinguishingvaginalacidphosphataseandseminalacidphosphataseusingisoelectricfocusing techniques.

Unit-2
Teaching Hours:15
Plant Poison
 

Identification and matching of various types of wood, timber varieties, seeds and leaves. Types of vegetablefibers and methods of their identification. Planktons and Diatoms- Forensic importance. Isolation of diatomfromwater,bodyorgansandtissues.Identificationofpollengrainsandstarchgrains.PaperPulpexamination.Identificationofpoisonousplantsofforensicsignificance,Abrusprecatorius,Aconitum,Argemonemexicana,Cannabissativa,Atropabelladonna,Erythroxylumcoco,Lathyrussativus,Manihotutilissima,Neriumindicum,Ricinuscommunis,Strychnosnuxvomica,Ergot,Opium,DhaturaandPsilocybinmushrooms.

Unit-3
Teaching Hours:15
Forensic Entomology
 

ForensicEntomology-History,significance,determinationoftimesincedeathDipteranslarvaldevelopment-lifecycleofblowfly,housefly,flesh-fly.Successionalcolonizationofbody,determiningwhether the body has been moved, body disturbance, presence and position wounds, linking suspect to thescene, identification of drugs and toxins from the insects and larvae feeding on the body, entomology as anevidentiarytoolinchildandseniorabusecasesandanimalabusecases,collectionandpreservationofentomologicalevidence.

Unit-4
Teaching Hours:15
Wildlife Forensics
 

IntroductionandimportanceofWildlife.Protectedandendangeredspeciesofanimalsandplants.Sanctuaries and their importance. Relevant provision of wildlife and environmental act. Types of wildlifecrimes.Differentmethods of killing andpoaching of wildlife animals, Illegaltrading inwildlife items, suchas skin, fur, bone, horn, teeth, flowers and plants. Identification of physical evidence pertaining to wildlifeforensics.Identificationof pugmarksofvariousanimals.

Text Books And Reference Books:

1.  Hosetti,B.B;“ConceptinWildlifeManagement”,DayaPublishingHouse,2005.

2.  Linarce,Adrian;“ForensicScienceinWildlifeInvestigation”,CRCPress,Taylor&Francis,2009.

3.  Baalu,T.R.;“TheWildlifeProtectionAct,1972”,NatarajPublication,2001.

Essential Reading / Recommended Reading

1.  UniversalPublication;“Wildlife(ProtectionAct,1972)”,UniversalPublication,2005.

2.  HeatherMillerCoyle;“ForensicBotany”,CRCPress,2005.

3.  X-RayManualbyWCCB,2013.

4.HouckM.Max&SiegelA.Jay,FundamentalsofForensicScience(3rdedition),AcademicPress,2015.8.RiversB.David&DahlemA.Gregory,TheScienceofForensicEntomology,Wiley-Blackwell,2014.9.StuartH.Barbara,ForensicAnalyticalTechniques,JohnWiley&Sons,2013.

10.GunnAlan,EssentialForensicBiology(3rdedition),JohnWiley,2019.

11.     LappasT.Nicholas&LappasM.Courtney,ForensicToxicology:PrinciplesandConcepts,ShirleyDecker-Lucke,2016.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%)

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

End semester examination : 100 Marks

MFS441C - MODERN TRENDS IN FINGERPRINT SCIENCE (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This course will help the students to learn advancement in fingerprints has facilitatedforensicinvestigation.Thispaperdealswithmorphologyandanatomyoffingerprintsandvariousdevelopmentaltechniques.Thiscourseprovidestheinformationaboutthirdleveldetailsincludingporoscopy and edgeoscopy and their utilization inpersonal identification.Thispaper willhelp studentstogetfamiliarwithadvancementin fingerprintcomputerizedapproach.

Course Outcome

CO1: This paper deals with morphology and anatomy of fingerprints and various developmental techniques.

CO2: This course provides the information about third level details including poroscopy and edgeoscopy and their utilization in personal identification.

CO3: This paper will help students to get familiar with advancement in fingerprint computerized approach.

CO4: This paper will help the students to learn about advancement and implementation of various computerized approach to establish the identity of an individual.

CO5: Students will learn the basic concepts of the mechanism of AFIS, basic applications of biometric.

Unit-1
Teaching Hours:15
Morphology and Anatomy of Fingerprint.
 

Introductionandrecapsulationtofingerprintscience,FunctionsofFingerprintsbureau,Fundamentalprinciples of fingerprint science, Anatomy of skin, biological significance of skin, Composition of sweat,secretaryglandseccrineglandsapocrine,glandssebaceousglandschemical composition,Ageof donor-variousage- groups,Composition oflatentprintresidues.

Unit-2
Teaching Hours:15
Development of Fingerprints
 

Iodine fuming, cyanoacrylate esters acceleration procedures, Post treatment procedures, Fluorescent andother chemical alternatives, Ninhydrin analogues, silver nitrate reagent, Special surfaces and situations:bloodyprints,tapeandstickysurfacesandskin,Postmortemfingerprintingtechniques.Latentprintenhancement by Laser and other alternate light sources: Using photo luminescent nano particles, basics of timegatedfingerprintdetection

Basics of phase resolved imaging, Fingerprint treatments: lanthanide based procedures, Photo luminescentfluorescence and phosphorescence, Use of Nano particles: cadmium, Zinc, Silver salts, Formation of silverphysical developer particles and preparation, Water and acid pretreatment, Multi metal deposition process,Colloidalgoldsolution,modifiedphysicaldeveloperenhancementtechniques,Digitalimagingmethods,optic methods,X ray,SEMmethods

Unit-3
Teaching Hours:15
Poroscopy and Edgeoscopy
 

IntroductiontoMolecularfingerprinting-importanceofthemoleculesdetectedfromthefingerprint residues-factorsinfluencingthem-identificationcharacteristicswiththemolecularconcentration,Importance of ridgeology and poroscopy- for individual identification-, Application of the pores dimensionsandridgedimensionsintheidentification-scopeofestablishingtheidentification –characteristicsandtraits,Applicationofedgeoscopy-personalidentificationusingfingerprints

Unit-4
Teaching Hours:15
Automated Fingerprint Identification System and other approaches
 

Introduction,emergingapplication,Systemarchitecture,sensing,fingerprintrepresentation

Minutiaefeatureextraction,orientation,estimation,segmentationsegmentation,ridgedetection,Ridgedetection, finger print matching enhancement, Challenge, system issues system evaluation, Other biometricmethodsofidentificationirisscan,retinalscan.,Cheiloscopy,palatoprints,earprintsetc.inforensicandothersciences.,Importanceofporoscopy,ridgeology/edgeoscopy,AnintroductiontoUIDAadhaaranditssignificance, ScopeofresearchonDNAfromfingerprintresidues

Text Books And Reference Books:

1.  DavidR.Ashbaugh;QuantitativeandQualitativeFrictionRidgeAnalysis,CRCPress(1999)

2.  E.RolandMenzel;FingerprintDetectionwithLasers,2ndEd.,MarcelDekker,Inc.USA(1999)

3.      JASeigel,P.JSaukooandGCKnupfer;EncyclopediaofForensicSciencesVol.I,IIandIII,Acad.Press2000.

4.      Menzel,ERoland;Fingerprintdetectionwithlasers,MarcelDekker,NY(1999)

Essential Reading / Recommended Reading

1.      JainLC;IntelligentBiometricTechniquesinFingerprintandfacerecognition,CRCPressOhio(1999)

2.        BridgesBC;CriminalInvestigation,Practicalfingerprinting,ThumbImpressions,HandwritingexperttestimonyopinionEvidence,UniversityBookAgency,Allahabad2000.

3.      Maltoni,Davide;Handbookoffingerprintrecognition,SpringerVerlag,NY,2003

4.      RathaNalini;AutomaticFingerprintrecognitionsystem,SpringerPub.,NY,2004.

5.  DaluzMosesHillary,FundamentalsofFingerprintAnalysis,Taylor&Francisgroup,2015.

6.  JohariK.C.,ForensicScienceIdentification ofFingerprints(1stedition),AsianLawHouse,2018.

7.  RamachandranR.,Scientifictechniquesincriminalinvestigation(2ndedition),KamalPublishers,2021.

8.                         RobinsonM.Edward,CrimescenePhotography(2ndedition),Academicpress,2010.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%)

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks 

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

End semester examination : 100 Marks

MFS442A - ANALYTICAL FORENSIC TOXICOLOGY (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to explain the conceptof introductiontoxicologicalanalysis of samples and its analytical techniques. It will also familiarize students’ alcohol toxication theirpreliminaryandinstrumental analysis. Thecourse also aimsto discuss the various applicationof animalpoison,identificationandexaminationprocess.Thissubjectwillfocusonagaseouspoison,signandsymptom,analysisof samplesandlegalaspects.

Course Outcome

CO1: To understand the concept of introduction of toxicological analysis of samples and its analytical techniques.

CO2: To understand and familiarize the students with alcohol toxication their preliminary and instrumental analysis.

CO3: To learn application of animal poison, identification and examination process.

CO4: To learn about various gaseous poison and food poison, sign and symptom, analysis of samples and legal aspects.

CO5: To understand the tools and techniques in forensic toxicology.

Unit-1
Teaching Hours:15
Introduction to toxicological analytical techniques
 

SamplesrequiredinToxicologicalanalysis:SelectionofPost-mortemsamplesandreferencetoparticularclassofpoison,Classesofsamples(BiologicalandNon-biological),Methodsofsamplecollection(LivingandDead person),choice ofpreservatives,containersandstorageconditions.Alternative specimens:Hairanalysis,Drugsinoralfluid,Detectionofdrugsinsweatetc.·           Extractiontechniques.TLC,HPTLC,HPLC,LC MS,GCMS, FTIRandAAS.

Unit-2
Teaching Hours:15
Alcohol Intoxication
 

AlcoholIntoxication&analysis:Relatedcases,PropertiesandtypesofAlcohols,Pharmacology,Toxicproperties and effects of alcohol. Chemical tests for alcohol in blood and urine including Breath AlcoholScreeningdevices,Methodofanalysisofsomealcoholicbeveragesinbiologicalmaterialsbychemicalmethods (Kozelka- Hine) and instrumental methods (GC), Legal context to drinking and driving. Analysis ofBeverages:Alcoholicandnon-alcoholicbeveragesandtheircomposition,Analysisofalcoholicbeveragesas per BIS and PFA Act, Detection and determination of ethanol, furfural, organic acids, aldehydes, chloralhydrate, methanol and ethylene glycol in liquors by color tests, TLC, GC, and GC-MS methods, Distinctionbetweenlicitandillicitliquors.FormatofReportWriting&CourtRoomTestimony:InformationrequiredbytheForensictoxicologist,PresentingfindingsinaReportformat.

Unit-3
Teaching Hours:15
Animal, Plants and microbial Poison
 

AnimalPoisons:Insectsandanimaltoxinsandtheirexamination,CompositionofSnakevenoms,Sitesandmodeofaction,Effectonthebodyasawhole,andtestsforidentifications.Plantpoisons:Classificationandcharacteristics,methodofextractionandstrippingofplantpoisonsinmatricesandanalysisbychemicalandvariousinstrumental techniques.

Unit-4
Teaching Hours:15
Gaseous and Food Poison
 

GaseousPoisoning:CarbonMonoxide,HydrogenCyanideandPhosphinegas,significance,signsandsymptoms, methods of diagnosis, different samples and procedures of sampling, tests for identification, andInstrumentalanalysis.

Food Poisoning, responsible factors;chemicaland bacterial, Sign and symptoms of food poisoning,collection and preservation of evidence material, extraction and isolation of poison from food material,biological material, detection and identification by color test and Instrumental techniques. Adulterations infood andtheiranalysisandFASSI.

Text Books And Reference Books:

1.      Casarett&Doll.Toxicology;theBasicScienceofpoisons.Taylor&FrancisPublication,2003.

2.      RiversB.David&DahlemA.Gregory,TheScienceofForensicEntomology,Wiley-Blackwell,2014.

3.      SkoogA.Douglas,HollerJamesF.&CrouchR.Stanley,PrinciplesofInstrumentalAnalysis(7th

edition),Cengage,2018

Essential Reading / Recommended Reading

1.      RamachandranR.,Scientifictechniquesincriminalinvestigation(2ndedition),KamalPublishers,2021.

2.      HouckM.Max,ForensicBiology:AdvancedForensicScienceSeries(1stedition),Academicpress,2015.

3.      SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015.

4.      LappasT.Nicholas&LappasM.Courtney,ForensicToxicology:PrinciplesandConcepts,ShirleyDecker-Lucke,2016.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%) CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

End semester examination : 100 Marks 

MFS442B - THEORETICAL AND PRACTICAL ASPECTS OF BIOLOGICAL EVIDENCES (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of this course is to explain the concept of introduction hairs its analytical techniques and DNAtyping. It will also familiarize students’ forensic characterization of blood their preliminary and instrumentalanalysis.Thecoursealsoaimstodiscussthevariousapplicationofbiologicalfluids,identificationandexaminationprocess.Thissubjectwillfocusonserologicaltechniquesandvariousadvancedtechniques.

Course Outcome

CO1: To identify examination various biological fluids and DNA typing.

CO2: To understand forensic characterization of blood formation and forensic significance.

CO3: To Identify and determine DNA typing of biological fluids.

CO4: To learn about various serological techniques and species identification.

CO5: To correlate the evidence to resolve a crime scene.

Unit-1
Teaching Hours:15
Evidence Collection Method
 

Evidencecollection-Guidelines,MethodsofCollectionofTraceEvidenceatCrimeScenes,Typesofevidence-Chemistryandproperties,PresumptiveandConfirmatorytests,Individualization,Preservationmethods of collected evidence, Digital evidence collection in cyber security .Indian Evidence act, Evidencecorrelationsand forensiclawrequirements.

Unit-2
Teaching Hours:15
Forensic Hair Characterization
 

Morphologyandtypesofhairs,importance,nature,location,collection,evaluationandtestsfortheiridentification: Species of origin, variation in different major population groups, somatic origin. Methods ofIndividualization:

Unit-3
Teaching Hours:15
Forensic Characterization of Biological samples-Body fluids
 

ForensicCharacterizationofSemen:Formation,Composition,Morphologyofspermatozoa,forensicsignificance,PresumptiveandConfirmatorytests(includingAzo-spermicsemenstains)Individualization(BloodGrouping,seminalfluidisozymestyping,forensicsignificance,CollectionandanalysisofevidencesinRapecases,Markergenesfor bodyfluidanalysis,

 

Unit-4
Teaching Hours:15
Techniques of Biological Fluids
 

Semen, saliva and other body fluids by Absorption- inhibition, Absorption-elution and mixed agglutinationtechniques,determinationofsecretor/non-secretorstatus.Primarybindingassays(ELISA,Immunochromatographicassays), Secondary bindingassays(Precipitationbased assays-ImmunodiffusionandelectrophoreticmethodsforspeciesIdentification,Agglutinationbasedassays-Directagglutinationassay, Passive agglutinationassay). Different methods for isolation of microorganisms from forensic sampleslike vomit,stool,stomach washandresidualfood.

Text Books And Reference Books:

1.  Race,R.R,andSanger,R.BloodGroupsinMan.BlackwellScientific,Oxford.

2.  LiR.ForensicBiology,Taylor&FrancisGroupLLC,2008.

3.  Robertson.ForensicexaminationofHair.Francis&Taylor,USA,1999

4.SafersteinRichard,Criminalistics:AnIntroductiontoForensicScience(11thedition),Pearson,2015

Essential Reading / Recommended Reading

1.ButlerM.John,FundamentalsofForensicDNATyping,AcademicPress,2010.

2.SennR.David&WeemsA.Richard,ManualofForensicOdontology(5thedition),Taylor&Francisgroup,2013.

3.  HouckM.Max&SiegelA.Jay,FundamentalsofForensicScience(3rdedition),AcademicPress,2015.8.KumarK.,ForensicBallisticsinCriminalJustice(1stedition),EasternBookCompany,2015.

9.     LaboratoryProcedureManual-ForensicSerology.DirectorateofForensicScience,MHA,NewDelhi2005.

10.LaboratoryProcedureManual–DNAProfiling,DirectorateofForensicScience,MHA,NewDelhi.2005

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%)

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

End semester examination : 100 Marks

MFS442C - ADVANCED QUESTIONED DOCUMENTS (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Advancementinquestioneddocumentsexaminationhasledtheinvestigationparabove the traditional methods of examination. The objective of this course is to disseminate the knowledgeaboutvarioustypesofforensicdocumentsincludinghandwritten,forgedandholographicdocuments.Italso aimstodiscusstheprinciplesofhandwriting,itsidentification,toolsneededforforensicdocumentexamination&theiruseinforensic science. Thecoursewildexplains thetypewritten,printed documentsandforgerydetection techniquestostudents

Course Outcome

CO1: Students will learn the comparative features of ink and paper

CO2: Students will have detailed idea about the admitted writing and specimen writing

CO3: Students will get detailed knowledge about the security features of government authorised documents

CO4: Students will understand the impact of alchohol, drugs and other substance abuse in handwritings

CO5: Students will learn about, Simulated Forgery and traced forgery

Unit-1
Teaching Hours:15
Handwritten Documents
 

Handwriting: Writing Systems, Style orNaturalcharacteristics in handwriting, Effect of various writinginstruments, age and illness on handwriting characteristics. Forgery: Introduction, Classes of forgery, theirexaminationandcomparison.ExaminationofnumeralandinitialsDisguiseinhandwriting,anonymousletters,Handednessandambidexterity.Composition ofink,paper and theirexamination,Comparison ofPrintedmatter:conventionalPrintingmethods-letterpress,offset,IntaglioandLithography.

Unit-2
Teaching Hours:15
Document Examination
 

Natureandproblemsofdocumentexamination,Careandhandlingofdocuments,classificationofdocuments, Procurements of standards- admitted / specimen writings, Handling and packing of documents,Preliminaryexaminationofdocuments,Principlesofhandwriting&signatureidentification.,PhysicalmatchingofDocuments.DigitalDocumentsandWatermarks(authenticationofdigitaldocuments).

Unit-3
Teaching Hours:15
Alteration and Obliteration of documents
 

Examinationofalterations,erasers,overwriting,additionandobliterations.Deciphermentofsecret,indentedandcharreddocuments,Photographyofquestioneddocuments.   Determinationofsequenceofstrokes,Examinationofcounterfeitcurrencynotes,passport,creditcard,visa,sealandothermechanicalimpressions,Examinationoftypescripts,xeroxand  computerprintoutsInstrumentaltechniquesusedfordocumentexaminations

Unit-4
Teaching Hours:15
Personality Assessment from documents
 

Evaluationofpersonality andpsychologicaltraitsfromhandwriting,Evolutionaryphasesinhandwriting,self-imageconcept,letterspecifics,,paceofwriting,rhythmandformquality,connectednessanddisconnectedness,effectofagefactorinhandwritingandvariationsinhandwritingcharacteristics,effectofalcoholanddrugsonhandwritingcharacteristics/signatures,ForensicStylistics.

Text Books And Reference Books:

1.  SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015.

2.  StuartH.Barbara,ForensicAnalyticalTechniques,JohnWiley&Sons,2013.

3.  Subrahmanyam,N.&LalB;AtextBookofOptics,S.Chand&Company,NewDelhi,2004.


4.    SkoogA.Douglas,HollerJamesF.&CrouchR.Stanley,PrinciplesofInstrumentalAnalysis(7th

edition),Cengage,2018

Essential Reading / Recommended Reading

 

1.  Khandpur,R.S;HandbookofAnalyticalInstruments,TataMcGrawHillPub.Co.NewDelhi,2004.

2.  SafersteinRichard,Criminalistics:AnIntroductiontoForensicScience(11thedition),Pearson,2015

3.  DavidJ.Thomas&LewisM.James,ForensicOdontologyPrinciplesandPractice,MicaHaley,2018

 

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%)

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

End semester examination : 100 Marks

MFS451A - FORENSIC DRUGS ANALYSIS AND ANALYTICAL FORENSIC TOXICOLOGY LAB (2022 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This course will help to developing an understanding of identification of various drugsandtheiranalysis,Instrumentalanalysisofcommondrugs,Identificationandextractionofpoisonspresentinvisceraandtodeterminethetoxicityofplantpoisonandimplementationofvariousanalyticaltechniques.

Course Outcome

CO1: To identify and determine various drugs and their analysis

CO2: To understand on Instrumental analysis of common drugs.

CO3: To Identify and determine poisons present in viscera.

CO4: To learn and the toxicity of plant poison and implementation of various analytical techniques.

CO5: To understand the morphological features of commonly available plant poison.

Unit-1
Teaching Hours:60
Forensic Drug Analysis
 

1.  Identificationofcommonprecursors.

2.   Identificationofnarcoticdrugs:opiumandalkaloids,morphineandheroin,cannabisbycolortestTLC,andinstrumentaltechniques.

3.  AnalysisofCannabisbycolortest,TLC/HPTLC

4.  DeterminationofbarbituratebyUV-visibleSpectrophotometricmethod.

5.  DeterminationofmorphineandheroininagivensamplebyUV-visiblespectrometer/LC.

6.  DeterminationofmorphineandheroininagivensamplebyGLCmethod.

7.  Identificationofketaminebycolortest&TLC.

8.  Analysisofstimulantsbycolortests,TLC/HPTLC.

9.  IdentificationofunknownseizedNDPSDrugbychemicalmethodsandInstrumentaltechniques.

10.  Extractionofdrugsfromhairsample.

11.  StudyofFTIRspectraofbenzodiazepines&Narcotics.

Unit-2
Teaching Hours:60
Analytical Forensic Toxicology
 

1.  Analysisofvisceraforvolatilepoisons(OrganicandInorganic)byConwayapparatus.

2.    Detectionandidentificationofmetallicpoisonsinvisceraandfoodmaterialbychemicaltestandinstrumentaltechnique.

3.  AnalysisofvisceraforpesticidebycolortestTLC/HPTLCandUV-visiblespectrometrymethod.

4.  Determineconcentrationofalcoholinbloodandurinesample.

5.  Systematicextraction,andidentificationofnon–volatiledrugsandplantpoisonsbyvarioustechniques.

6.   Identificationofcommonplantpoisonsopiumandalkaloids,Kaner,DhaturaandNuxVomica,Aconitebycolortest,cannabisandinstrumentaltechniques.

7.  Detectionandidentificationofmajormetabolitesofethanol,methanol,parathion,carbryl.

8.    Determinationofphosphineinaluminumphosphideandzincphosphideinviscerabychemicalandinstrumentalanalysis.

9.    Identificationofpsychotropicdrugs-barbiturates,benzodiazepines&narcoticsinbiologicalfluidsbycolor test,TLC/HPTLC andinstrumentaltechniques.

10.  Morphologicalanalysisofcommonplantpoisons

Text Books And Reference Books:

1.      Casarett&Doll.Toxicology;theBasicScienceofpoisons.Taylor&FrancisPublication,2003.

2.      RiversB.David&DahlemA.Gregory,TheScienceofForensicEntomology,Wiley-Blackwell,2014.

3.      SkoogA.Douglas,HollerJamesF.&CrouchR.Stanley,PrinciplesofInstrumentalAnalysis(7th

edition),Cengage,2018

Essential Reading / Recommended Reading

1.      RamachandranR.,Scientifictechniquesincriminalinvestigation(2ndedition),KamalPublishers,2021.

2.      HouckM.Max,ForensicBiology:AdvancedForensicScienceSeries(1stedition),Academicpress,2015.

3.      SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015.

4.      LappasT.Nicholas&LappasM.Courtney,ForensicToxicology:PrinciplesandConcepts,ShirleyDecker-Lucke,2016.

Evaluation Pattern

CIA Evaluation Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination (External examiners) – 100 marks

MFS451B - FORENSIC BIOLOGY AND WILDLIFE FORENSICS AND THEORETICAL AND PRACTICAL ASPECTS OF BIOLOGICAL EVIDENCES LAB (2022 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Thiscoursewillhelptodevelopinganunderstandingofidentificationofvariousbiologicalfluidsandtheirexaminations,isolationandquantificationofDNAfromblood,identificationand extractionofproteinspresentinbiologicalsamplesandtoimplementthedifferenttechniquesforproteinestimationandanalysis.

Course Outcome

CO1: To identify various biological fluids and their forensic examinations.

CO2: Isolation and quantification of DNA and protein from the forensic samples.

CO3: Students will be able to understand the identification, examination and analysis forensic plant materials like pollen, fibre etc.

CO4: Students will be able to understand the different chromatographic techniques in analysis of plant poisons.

CO5: Students will be able to understand the identification, Examination and analysis of wild life materials like skin, fur, bones, nails etc. which will be useful for the wild life forensic investigation

Unit-1
Teaching Hours:120
Practicals
 

1.      Differentstainingproceduresforcellularorganelles.

2.      Physical,biochemicalandspectrophotometricexaminationofbloodstains.

3.        Examinationofseminalstainsbycrystaltests,biochemicalandmicroscopicalanalysis.

4.      Examinationofsalivaanditsstains.

5.      Determinationoforiginofspeciesfrombiologicalfluids.

6.      CollectionandextractionofDNAusingFTAcard.

7.      IsolationofDNAfrombloodanditsquantification.

8.      Experimentsonelectrophoresisofredcellisoenzymese.g.PGM,GLO-I,EsD,EAP.

9.      Extractionofproteinsfromvariousbiologicalsamples.

10.    Proteinestimationbydifferenttechniques

11.    SDS-PAGEforproteinanalysis

12.    DNAextractionfromvariousforensicsamples

13.    ConstructionofPhylogenetictreefromnucleotideandproteinsequences.

14.    AnalysisofproteinstructureusingRASMOL

15.    Morphologicalandanatomicalexaminationofpoisonousplantsgiveninthesyllabus(any5)

16.    Extractionandcharacterizationofphytochemicalspresentinthepoisonousplantsinthesyllabus(fromany3 plants)usingchromatography techniques.

17.    Identificationofwildlifematerialssuchasskin,fur,bones,nails,horn,teeth,flowersandplants

18.    Examinationoffur,nails,horn,teeth

19.    ExaminationofhairofdifferentanimalssuchasDogs,Cats,Cow,Horse,Goatsetc.

20.    Determinationofhumanhairmorphology.

21.    Studyofpugmarksofanimals

22.    PreparationofpermanentslidesbyusingmacerationtechniqueofvariousforensicmaterialofPlantorigin

23.    Studyofpollengrainsandsporesofforensicsignificance.

24.    Morphological&microscopicexaminationoffibres.

25.    Microscopicandchemicalcomparisonofpaperpulp.

26.    Identificationofdiatoms

27.    Identificationofstarchgranules.

28.    Commonstainingtechniquesandlaboratoryexercisesforidentificationofdifferentplantcelltypes.

Text Books And Reference Books:

1.  Race,R.R,andSanger,R.BloodGroupsinMan.BlackwellScientific,Oxford.

2.  LiR.ForensicBiology,Taylor&FrancisGroupLLC,2008.

3.  Robertson.ForensicexaminationofHair.Francis&Taylor,USA,1999

4.SafersteinRichard,Criminalistics:AnIntroductiontoForensicScience(11thedition),Pearson,2015

Essential Reading / Recommended Reading

1.ButlerM.John,FundamentalsofForensicDNATyping,AcademicPress,2010.

2.SennR.David&WeemsA.Richard,ManualofForensicOdontology(5thedition),Taylor&Francisgroup,2013.

3.  HouckM.Max&SiegelA.Jay,FundamentalsofForensicScience(3rdedition),AcademicPress,2015.8.KumarK.,ForensicBallisticsinCriminalJustice(1stedition),EasternBookCompany,2015.

9.     LaboratoryProcedureManual-ForensicSerology.DirectorateofForensicScience,MHA,NewDelhi2005.

10.LaboratoryProcedureManual–DNAProfiling,DirectorateofForensicScience,MHA,NewDelhi.2005

Evaluation Pattern

CIA Evaluation Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination (External examiners) – 100 marks

MFS451C - PRACTICAL OF QUESTIONED DOCUMENTS AND FINGERPRINTS (2022 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

thiscoursewillhelptogainknowledgeaboutdevelopinganunderstandingofexamination of several questioned documents, Instrumental analysis of questioned documents and ageing ofpapers,Development(Physicaldeveloper)oflatentfingerprintspresentonvarioussurfacesandidentificationofcharacteristics,Implementationofvariouschemicalmethodsfordevelopmentoffingerprintsrecovered inseveralconditions.

Course Outcome

CO1: Students will have practical knowledge on free hand forgery and traced forgery

CO2: Students can differentiate between types of inks through TLC examination

CO3: Students can classify the finger prints based on different types of finger print classification system

CO4: Students will be able to analyse questioned documents and ageing of papers

CO5: Students will learn different physical and chemical development methods of finger prints

Unit-1
Teaching Hours:60
Questioned Documents
 

1.      Tostudytheforgedsignatures(free-handforgeryandtracedforgery)

2.      Tostudytheinitialsofhandwrittendocuments

3.      ToperformTLCofwritinginks,tonersandinkjetinksandwritingpapers.

4.      Tostudysequenceofintersectingstrokes.

5.        TostudythePhotocopieddocuments(Physicalexamination).

6.        TostudytheLaserprinteddocuments(Physicalexamination).

7.        TostudytheInkjetprinteddocuments(Physicalexamination).

8.        ToperformCyno-acrylatemethodtodeveloplatentfingerprintsoverdocuments.

9.      ToclassifythefingerprintsfromPrimaryclassificationtokeyclassification.

10.  ToperformSPRmethodtodeveloplatentprints.

11.  ExaminationofinkbyGCMS

12.  ExaminationofinkbyLCMS


13.  Examinationofageingofpaper

14.  ExaminationofStamp

15.  ExaminationofRubberStampImpressions

16.  ExaminationofdocumentsbyVSC5000

17.  InkanalysisbyIonexchangemethod

18.  Documentsexaminationbyconfocalmicroscope.

19.  Preservation of Charred documents.

20.  Examination of Fake plastics currency.

21.  Establishment of identity based on pores and edges of fingerprints.

22.  Development of fingerprints on various surfaces by advanced techniques.

Unit-2
Teaching Hours:60
Fingerprints
 

1.      Analysisoffingerprintswithmicroscopictechniquesfortheridgedimensionswiththecompleteidentificationprofiling

2.      Comparisonofmales’andfemales’fingerprintswiththespecificreferencetotheridgedimensions

3.      DevelopmentoflatentfingerprintsusingNinhydrin.

4.      Developmentoflatentfingerprintsusingiodinefuming

5.      DevelopmentoflatentfingerprintsusingNanoparticles

6.      UnderstandingAFISmethodoffingerprintsanalysis

7.      Palato-printsandidentificationbymicroscopicexamination

8.      Cheiloscopy;microscopicandmacroscopicexamination

9.      Importanceofmolecularfingerprintswithspecialreferencetofingerprintresiduesusinginstrumentalmethod

Text Books And Reference Books:

1.  SiegelA.Jay&MirakovitsKathy,ForensicSciencethebasic(3rdedition),CRCPress,2015.

2.  StuartH.Barbara,ForensicAnalyticalTechniques,JohnWiley&Sons,2013.

3.  Subrahmanyam,N.&LalB;AtextBookofOptics,S.Chand&Company,NewDelhi,2004.


4.    SkoogA.Douglas,HollerJamesF.&CrouchR.Stanley,PrinciplesofInstrumentalAnalysis(7th

edition),Cengage,2018

Essential Reading / Recommended Reading

 

1.  Khandpur,R.S;HandbookofAnalyticalInstruments,TataMcGrawHillPub.Co.NewDelhi,2004.

2.  SafersteinRichard,Criminalistics:AnIntroductiontoForensicScience(11thedition),Pearson,2015

3.  DavidJ.Thomas&LewisM.James,ForensicOdontologyPrinciplesandPractice,MicaHaley,2018

 

Evaluation Pattern

 CIA Evaluation Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End semester Examination (External examiners) – 100 marks

MFS481A - DISSERTATION (2022 Batch)

Total Teaching Hours for Semester:0
No of Lecture Hours/Week:0
Max Marks:150
Credits:12

Course Objectives/Course Description

 

 

The main objective of this course is the awareness and understanding of students in industrial research.

Course Outcome

CO1: To learn and utilize the experience for future endeavors.

CO2: Students will gain experience and practical knowledge.

CO3: Students can utilize advance techniques for research and innovation

CO4: Industrial experience will help the students in skills development

Unit-1
Teaching Hours:0
NA
 

NA

Text Books And Reference Books:

As per requirement of project

Essential Reading / Recommended Reading

As per requirement of project

Evaluation Pattern

Components of project i.e. Thesis, presentation and viva, publication. 

MFS481B - MAJOR PROJECT (2022 Batch)

Total Teaching Hours for Semester:0
No of Lecture Hours/Week:0
Max Marks:150
Credits:12

Course Objectives/Course Description

 

 

The main objective of this course is the awareness and understanding of students in  research.

Course Outcome

CO1: To work and gain experience on advance instruments and handling them

CO2: Students will conduct research on any topic from their field of specialization.

CO3: Development of skills and employability.

CO4: This project will help to attain certain objectives of innovation.

Unit-1
Teaching Hours:0
NA
 

NA

Text Books And Reference Books:

As per the requirement of project

Essential Reading / Recommended Reading

As per the requirement of project

Evaluation Pattern

Components of project i.e. Thesis, presentation and viva, publication.